The influence of side group modification in polyphosphazenes on hydrolysis and cell adhesion of blends with PLGA

Nicholas R. Krogman, Arlin L. Weikel, Katherine A. Kristhart, Syam P. Nukavarapu, Meng Deng, Lakshmi S. Nair, Cato T. Laurencin, Harry R. Allcock

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Polyphosphazenes have been synthesized with tris(hydroxymethyl)amino methane (THAM) side groups and with co-substituents glycine ethyl ester and alanine ethyl ester. The THAM side group was linked to the polyphosphazene backbone via the amino function. The three pendent hydroxyl functions on each THAM side group were utilized for hydrogen bonding association with poly(glycolic-lactic acid) (PLGA). Co-substitution of the polyphosphazene with both THAM and glycine or alanine ethyl esters was employed to avoid the insolubility of the single-substituent THAM-substituted polyphosphazenes. Both poly[(tris(hydroxymethyl)aminomethane)(ethyl glycinato)phosphazene] and poly[(tris(hydroxymethyl)aminomethane)(ethyl alanato)phosphazene] (1:1 ratio of side groups) were blended with PLGA (50:50) or PLGA (85:15). DSC analysis indicated miscible blend formation, irrespective of the detailed molecular structure of the polyphosphazene or the composition of PLGA in the blend. Hydrolysis studies of the polyphosphazene:PLGA (50:50) blends indicated that the PLGA component hydrolyzed more rapidly than the polyphosphazene. Primary osteoblast cell studies showed good cell adhesion to the polymer blends during 14 days, but subsequent limited cell spreading due to increased surface roughness as the two polymers eroded at different rates.

Original languageEnglish (US)
Pages (from-to)3035-3041
Number of pages7
JournalBiomaterials
Volume30
Issue number17
DOIs
StatePublished - Jun 1 2009

Fingerprint

glycolic acid
Cell adhesion
Lactic acid
Cell Adhesion
Methane
Hydrolysis
Lactic Acid
Esters
Tromethamine
Alanine
Amino acids
Polymers
Osteoblasts
Polymer blends
Hydrogen Bonding
Molecular Structure
Hydroxyl Radical
Glycine
Molecular structure
poly(lactic acid)

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Krogman, Nicholas R. ; Weikel, Arlin L. ; Kristhart, Katherine A. ; Nukavarapu, Syam P. ; Deng, Meng ; Nair, Lakshmi S. ; Laurencin, Cato T. ; Allcock, Harry R. / The influence of side group modification in polyphosphazenes on hydrolysis and cell adhesion of blends with PLGA. In: Biomaterials. 2009 ; Vol. 30, No. 17. pp. 3035-3041.
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abstract = "Polyphosphazenes have been synthesized with tris(hydroxymethyl)amino methane (THAM) side groups and with co-substituents glycine ethyl ester and alanine ethyl ester. The THAM side group was linked to the polyphosphazene backbone via the amino function. The three pendent hydroxyl functions on each THAM side group were utilized for hydrogen bonding association with poly(glycolic-lactic acid) (PLGA). Co-substitution of the polyphosphazene with both THAM and glycine or alanine ethyl esters was employed to avoid the insolubility of the single-substituent THAM-substituted polyphosphazenes. Both poly[(tris(hydroxymethyl)aminomethane)(ethyl glycinato)phosphazene] and poly[(tris(hydroxymethyl)aminomethane)(ethyl alanato)phosphazene] (1:1 ratio of side groups) were blended with PLGA (50:50) or PLGA (85:15). DSC analysis indicated miscible blend formation, irrespective of the detailed molecular structure of the polyphosphazene or the composition of PLGA in the blend. Hydrolysis studies of the polyphosphazene:PLGA (50:50) blends indicated that the PLGA component hydrolyzed more rapidly than the polyphosphazene. Primary osteoblast cell studies showed good cell adhesion to the polymer blends during 14 days, but subsequent limited cell spreading due to increased surface roughness as the two polymers eroded at different rates.",
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Krogman, NR, Weikel, AL, Kristhart, KA, Nukavarapu, SP, Deng, M, Nair, LS, Laurencin, CT & Allcock, HR 2009, 'The influence of side group modification in polyphosphazenes on hydrolysis and cell adhesion of blends with PLGA', Biomaterials, vol. 30, no. 17, pp. 3035-3041. https://doi.org/10.1016/j.biomaterials.2009.02.049

The influence of side group modification in polyphosphazenes on hydrolysis and cell adhesion of blends with PLGA. / Krogman, Nicholas R.; Weikel, Arlin L.; Kristhart, Katherine A.; Nukavarapu, Syam P.; Deng, Meng; Nair, Lakshmi S.; Laurencin, Cato T.; Allcock, Harry R.

In: Biomaterials, Vol. 30, No. 17, 01.06.2009, p. 3035-3041.

Research output: Contribution to journalArticle

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T1 - The influence of side group modification in polyphosphazenes on hydrolysis and cell adhesion of blends with PLGA

AU - Krogman, Nicholas R.

AU - Weikel, Arlin L.

AU - Kristhart, Katherine A.

AU - Nukavarapu, Syam P.

AU - Deng, Meng

AU - Nair, Lakshmi S.

AU - Laurencin, Cato T.

AU - Allcock, Harry R.

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N2 - Polyphosphazenes have been synthesized with tris(hydroxymethyl)amino methane (THAM) side groups and with co-substituents glycine ethyl ester and alanine ethyl ester. The THAM side group was linked to the polyphosphazene backbone via the amino function. The three pendent hydroxyl functions on each THAM side group were utilized for hydrogen bonding association with poly(glycolic-lactic acid) (PLGA). Co-substitution of the polyphosphazene with both THAM and glycine or alanine ethyl esters was employed to avoid the insolubility of the single-substituent THAM-substituted polyphosphazenes. Both poly[(tris(hydroxymethyl)aminomethane)(ethyl glycinato)phosphazene] and poly[(tris(hydroxymethyl)aminomethane)(ethyl alanato)phosphazene] (1:1 ratio of side groups) were blended with PLGA (50:50) or PLGA (85:15). DSC analysis indicated miscible blend formation, irrespective of the detailed molecular structure of the polyphosphazene or the composition of PLGA in the blend. Hydrolysis studies of the polyphosphazene:PLGA (50:50) blends indicated that the PLGA component hydrolyzed more rapidly than the polyphosphazene. Primary osteoblast cell studies showed good cell adhesion to the polymer blends during 14 days, but subsequent limited cell spreading due to increased surface roughness as the two polymers eroded at different rates.

AB - Polyphosphazenes have been synthesized with tris(hydroxymethyl)amino methane (THAM) side groups and with co-substituents glycine ethyl ester and alanine ethyl ester. The THAM side group was linked to the polyphosphazene backbone via the amino function. The three pendent hydroxyl functions on each THAM side group were utilized for hydrogen bonding association with poly(glycolic-lactic acid) (PLGA). Co-substitution of the polyphosphazene with both THAM and glycine or alanine ethyl esters was employed to avoid the insolubility of the single-substituent THAM-substituted polyphosphazenes. Both poly[(tris(hydroxymethyl)aminomethane)(ethyl glycinato)phosphazene] and poly[(tris(hydroxymethyl)aminomethane)(ethyl alanato)phosphazene] (1:1 ratio of side groups) were blended with PLGA (50:50) or PLGA (85:15). DSC analysis indicated miscible blend formation, irrespective of the detailed molecular structure of the polyphosphazene or the composition of PLGA in the blend. Hydrolysis studies of the polyphosphazene:PLGA (50:50) blends indicated that the PLGA component hydrolyzed more rapidly than the polyphosphazene. Primary osteoblast cell studies showed good cell adhesion to the polymer blends during 14 days, but subsequent limited cell spreading due to increased surface roughness as the two polymers eroded at different rates.

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