The inhibition of progesterone secretion and the regulation of cyclic nucleotides by atrial natriuretic factor in gonadotropin responsive murine Leydig tumor cells

Kailash N. Pandey, William J. Kovacs, Tadashi Inagami

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We have found that atrial natriuretic factor (ANF) has a profound effect on testicular cells in altering intracellular cyclic nucleotide levels as well as progesterone secretion. Using clonal cultured Leydig tumor cells we found that 1×10-8M ANF caused a two thousand-fold elevation in the accumulation of cellular cGMP and inhibited cAMP in treated cells by more than 90% as compared to the controls. ANF (1×10-8M) also significantly inhibited gonadotropin-stimulated accumulation of cAMP in response to bovine luteinizing hormone (bLH) or human chorionic gonadotropin (hCG). Gonadotropin-stimulated progesterone secretion was inhibited by ANF (1×10-10-1×10-9M) in these cultured Leydig tumor cells. Approximately 50% inhibition of progesterone secretion was observed at the peptide concentration of 1×10-9M.

Original languageEnglish (US)
Pages (from-to)800-806
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume133
Issue number2
DOIs
StatePublished - Dec 17 1985

Fingerprint

Leydig Cell Tumor
Cyclic Nucleotides
Atrial Natriuretic Factor
Gonadotropins
Progesterone
Tumors
Cells
Cultured Tumor Cells
Leydig Cells
Chorionic Gonadotropin
Luteinizing Hormone
Peptides

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{3ca8e3b40af94f2694d19377ae896efb,
title = "The inhibition of progesterone secretion and the regulation of cyclic nucleotides by atrial natriuretic factor in gonadotropin responsive murine Leydig tumor cells",
abstract = "We have found that atrial natriuretic factor (ANF) has a profound effect on testicular cells in altering intracellular cyclic nucleotide levels as well as progesterone secretion. Using clonal cultured Leydig tumor cells we found that 1×10-8M ANF caused a two thousand-fold elevation in the accumulation of cellular cGMP and inhibited cAMP in treated cells by more than 90{\%} as compared to the controls. ANF (1×10-8M) also significantly inhibited gonadotropin-stimulated accumulation of cAMP in response to bovine luteinizing hormone (bLH) or human chorionic gonadotropin (hCG). Gonadotropin-stimulated progesterone secretion was inhibited by ANF (1×10-10-1×10-9M) in these cultured Leydig tumor cells. Approximately 50{\%} inhibition of progesterone secretion was observed at the peptide concentration of 1×10-9M.",
author = "Pandey, {Kailash N.} and Kovacs, {William J.} and Tadashi Inagami",
year = "1985",
month = "12",
day = "17",
doi = "10.1016/0006-291X(85)90975-1",
language = "English (US)",
volume = "133",
pages = "800--806",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The inhibition of progesterone secretion and the regulation of cyclic nucleotides by atrial natriuretic factor in gonadotropin responsive murine Leydig tumor cells

AU - Pandey, Kailash N.

AU - Kovacs, William J.

AU - Inagami, Tadashi

PY - 1985/12/17

Y1 - 1985/12/17

N2 - We have found that atrial natriuretic factor (ANF) has a profound effect on testicular cells in altering intracellular cyclic nucleotide levels as well as progesterone secretion. Using clonal cultured Leydig tumor cells we found that 1×10-8M ANF caused a two thousand-fold elevation in the accumulation of cellular cGMP and inhibited cAMP in treated cells by more than 90% as compared to the controls. ANF (1×10-8M) also significantly inhibited gonadotropin-stimulated accumulation of cAMP in response to bovine luteinizing hormone (bLH) or human chorionic gonadotropin (hCG). Gonadotropin-stimulated progesterone secretion was inhibited by ANF (1×10-10-1×10-9M) in these cultured Leydig tumor cells. Approximately 50% inhibition of progesterone secretion was observed at the peptide concentration of 1×10-9M.

AB - We have found that atrial natriuretic factor (ANF) has a profound effect on testicular cells in altering intracellular cyclic nucleotide levels as well as progesterone secretion. Using clonal cultured Leydig tumor cells we found that 1×10-8M ANF caused a two thousand-fold elevation in the accumulation of cellular cGMP and inhibited cAMP in treated cells by more than 90% as compared to the controls. ANF (1×10-8M) also significantly inhibited gonadotropin-stimulated accumulation of cAMP in response to bovine luteinizing hormone (bLH) or human chorionic gonadotropin (hCG). Gonadotropin-stimulated progesterone secretion was inhibited by ANF (1×10-10-1×10-9M) in these cultured Leydig tumor cells. Approximately 50% inhibition of progesterone secretion was observed at the peptide concentration of 1×10-9M.

UR - http://www.scopus.com/inward/record.url?scp=0022396393&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022396393&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(85)90975-1

DO - 10.1016/0006-291X(85)90975-1

M3 - Article

C2 - 3002360

AN - SCOPUS:0022396393

VL - 133

SP - 800

EP - 806

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -