The Interaction of NSBP1/HMGN5 with Nucleosomes in Euchromatin Counteracts Linker Histone-Mediated Chromatin Compaction and Modulates Transcription

Mark Rochman, Yuri Postnikov, Sarah Correll, Cedric Malicet, Stephen Wincovitch, Tatiana S. Karpova, James G. McNally, Xiaolin Wu, Nina A. Bubunenko, Sergei Grigoryev, Michael Bustin

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Structural changes in specific chromatin domains are essential to the orderly progression of numerous nuclear processes, including transcription. We report that the nuclear protein NSBP1 (HMGN5), a recently discovered member of the HMGN nucleosome-binding protein family, is specifically targeted by its C-terminal domain to nucleosomes in euchromatin. We find that the interaction of NSBP1 with nucleosomes alters the compaction of cellular chromatin and that in living cells, NSBP1 interacts with linker histones. We demonstrate that the negatively charged C-terminal domain of NSBP1 interacts with the positively charged C-terminal domain of H5 and that NSBP1 counteracts the linker histone-mediated compaction of a nucleosomal array. Dysregulation of the cellular levels of NSBP1 alters the transcription level of numerous genes. We suggest that mouse NSBP1 is an architectural protein that binds preferentially to euchromatin and modulates the fidelity of the cellular transcription profile by counteracting the chromatin-condensing activity of linker histones.

Original languageEnglish (US)
Pages (from-to)642-656
Number of pages15
JournalMolecular cell
Volume35
Issue number5
DOIs
StatePublished - Sep 11 2009

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Euchromatin
Nucleosomes
Histones
Chromatin
HMGN Proteins
Nuclear Proteins
Carrier Proteins
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Rochman, Mark ; Postnikov, Yuri ; Correll, Sarah ; Malicet, Cedric ; Wincovitch, Stephen ; Karpova, Tatiana S. ; McNally, James G. ; Wu, Xiaolin ; Bubunenko, Nina A. ; Grigoryev, Sergei ; Bustin, Michael. / The Interaction of NSBP1/HMGN5 with Nucleosomes in Euchromatin Counteracts Linker Histone-Mediated Chromatin Compaction and Modulates Transcription. In: Molecular cell. 2009 ; Vol. 35, No. 5. pp. 642-656.
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Rochman, M, Postnikov, Y, Correll, S, Malicet, C, Wincovitch, S, Karpova, TS, McNally, JG, Wu, X, Bubunenko, NA, Grigoryev, S & Bustin, M 2009, 'The Interaction of NSBP1/HMGN5 with Nucleosomes in Euchromatin Counteracts Linker Histone-Mediated Chromatin Compaction and Modulates Transcription', Molecular cell, vol. 35, no. 5, pp. 642-656. https://doi.org/10.1016/j.molcel.2009.07.002

The Interaction of NSBP1/HMGN5 with Nucleosomes in Euchromatin Counteracts Linker Histone-Mediated Chromatin Compaction and Modulates Transcription. / Rochman, Mark; Postnikov, Yuri; Correll, Sarah; Malicet, Cedric; Wincovitch, Stephen; Karpova, Tatiana S.; McNally, James G.; Wu, Xiaolin; Bubunenko, Nina A.; Grigoryev, Sergei; Bustin, Michael.

In: Molecular cell, Vol. 35, No. 5, 11.09.2009, p. 642-656.

Research output: Contribution to journalArticle

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