The isolation and properties of the dimeric subunit of Concanavalin A

John H. Pazur, Michael D. Perloff, Adam R. Frymoyer, Carolyn J.P. Jensen, Holly Micolochick, Andrea Marie Mastro

Research output: Contribution to journalArticle

Abstract

Concanavalin A (Con A) was dissociated into dimeric and monomeric subunits by incubation at 37°C in acetate buffer of pH 3.8 containing 0.5% sodium dodecyl sulfate. The dimer was isolated in pure form by a density gradient ultracentrifugation method. Several properties of the dimer were determined including the formation of a precipitin with anti-Con A antibodies, the molecular weight, the lack of a binding site for glycogen, the lack of mitogenic activity for spleen lymphocytes, and the lack of inhibition by α-methyl D-glucoside. The latter findings differ from results reported by other investigators.

Original languageEnglish (US)
Pages (from-to)353-359
Number of pages7
JournalJournal of Protein Chemistry
Volume19
Issue number5
DOIs
StatePublished - Dec 1 2000

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Concanavalin A
Dimers
Precipitins
Lymphocytes
Ultracentrifugation
Sodium dodecyl sulfate
Binding sites
Glycogen
Antibodies
Sodium Dodecyl Sulfate
Buffers
Acetates
Spleen
Molecular Weight
Molecular weight
Binding Sites
Research Personnel
methylglucoside
Glucosides

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Pazur, J. H., Perloff, M. D., Frymoyer, A. R., Jensen, C. J. P., Micolochick, H., & Mastro, A. M. (2000). The isolation and properties of the dimeric subunit of Concanavalin A. Journal of Protein Chemistry, 19(5), 353-359. https://doi.org/10.1023/A:1026431329188
Pazur, John H. ; Perloff, Michael D. ; Frymoyer, Adam R. ; Jensen, Carolyn J.P. ; Micolochick, Holly ; Mastro, Andrea Marie. / The isolation and properties of the dimeric subunit of Concanavalin A. In: Journal of Protein Chemistry. 2000 ; Vol. 19, No. 5. pp. 353-359.
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Pazur, JH, Perloff, MD, Frymoyer, AR, Jensen, CJP, Micolochick, H & Mastro, AM 2000, 'The isolation and properties of the dimeric subunit of Concanavalin A', Journal of Protein Chemistry, vol. 19, no. 5, pp. 353-359. https://doi.org/10.1023/A:1026431329188

The isolation and properties of the dimeric subunit of Concanavalin A. / Pazur, John H.; Perloff, Michael D.; Frymoyer, Adam R.; Jensen, Carolyn J.P.; Micolochick, Holly; Mastro, Andrea Marie.

In: Journal of Protein Chemistry, Vol. 19, No. 5, 01.12.2000, p. 353-359.

Research output: Contribution to journalArticle

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Pazur JH, Perloff MD, Frymoyer AR, Jensen CJP, Micolochick H, Mastro AM. The isolation and properties of the dimeric subunit of Concanavalin A. Journal of Protein Chemistry. 2000 Dec 1;19(5):353-359. https://doi.org/10.1023/A:1026431329188