The isolation of structural genes from libraries of eucaryotic DNA

Tom Maniatis, Ross C. Hardison, Elizabeth Lacy, Joyce Lauer, Catherine O'Connell, Diana Quon, Gek Kee Sim, Argiris Efstratiadis

Research output: Contribution to journalArticlepeer-review

1028 Scopus citations

Abstract

We present a procedure for eucaryotic structural gene isolation which involves the construction and screening of cloned libraries of genomic DNA. Large random DNA fragments are joined to phage lambda vectors by using synthetic DNA linkers. The recombinant molecules are packaged into viable phage particles in vitro and amplified to establish a permanent library. We isolated structural genes together with their associated sequences from three libraries constructed from Drosophila, silkmoth and rabbit genomic DNA. In particular, we obtained a large number of phage recombinants bearing the chorion gene sequence from the silkmoth library and several independent clones of β-globin genes from the rabbit library. Restriction mapping and hybridization studies reveal the presence of closely linked β-globin genes.

Original languageEnglish (US)
Pages (from-to)687-701
Number of pages15
JournalCell
Volume15
Issue number2
DOIs
StatePublished - Oct 1978

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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