The jiaoyao1 mutant is an allele of korrigan1 that abolishes endoglucanase activity and affects the organization of both cellulose microfibrils and microtubules in Arabidopsis

Lei Lei, Tian Zhang, Richard Strasser, Christopher M. Lee, Martine Gonneau, Lukas Mach, Samantha Vernhettes, Seong H. Kim, Daniel J. Cosgrove, Shundai Li, Ying Gu

Research output: Contribution to journalArticle

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Abstract

In higher plants, cellulose is synthesized by plasma membrane-localized cellulose synthase complexes (CSCs). Arabidopsis thaliana GH9A1/KORRIGAN1 is a membrane-bound, family 9 glycosyl hydrolase that is important for cellulose synthesis in both primary and secondary cell walls. Most previously identified korrigan1 mutants show severe phenotypes such as embryo lethality; therefore, the role of GH9A1 in cellulose synthesis remains unclear. Here, we report a novel A577V missense mutation, designated jiaoyao1 (jia1), in the second of the glycosyl hydrolase family 9 active site signature motifs in GH9A1. jia1 is defective in cell expansion in dark-grown hypocotyls, roots, and adult plants. Consistent with its defect in cell expansion, this mutation in GH9A1 resulted in reduced cellulose content and reduced CSC velocity at the plasma membrane. Green fluorescent protein-GH9A1 is associated with CSCs at multiple locations, including the plasma membrane, Golgi, trans-Golgi network, and small CESA-containing compartments or microtubule-associated cellulose synthase compartments, indicating a tight association between GH9A1 and CSCs. GH9A1A577V abolishes the endoglucanase activity of GH9A1 in vitro but does not affect its interaction with CESAs in vitro, suggesting that endoglucanase activity is important for cellulose synthesis. Interestingly, jia1 results in both cellulose microfibril and microtubule disorganization. Our study establishes the important role of endoglucanase in cellulose synthesis and cellulose microfibril organization in plants.

Original languageEnglish (US)
Pages (from-to)2601-2616
Number of pages16
JournalPlant Cell
Volume26
Issue number6
DOIs
StatePublished - Jul 2014

Fingerprint

Microfibrils
Cellulase
Arabidopsis
endo-1,4-beta-glucanase
Cellulose
Microtubules
microtubules
cellulose synthase
cellulose
Alleles
alleles
mutants
synthesis
plasma membrane
glycosidases
Cell Membrane
Hydrolases
trans-Golgi Network
Hypocotyl
missense mutation

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Lei, Lei ; Zhang, Tian ; Strasser, Richard ; Lee, Christopher M. ; Gonneau, Martine ; Mach, Lukas ; Vernhettes, Samantha ; Kim, Seong H. ; Cosgrove, Daniel J. ; Li, Shundai ; Gu, Ying. / The jiaoyao1 mutant is an allele of korrigan1 that abolishes endoglucanase activity and affects the organization of both cellulose microfibrils and microtubules in Arabidopsis. In: Plant Cell. 2014 ; Vol. 26, No. 6. pp. 2601-2616.
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abstract = "In higher plants, cellulose is synthesized by plasma membrane-localized cellulose synthase complexes (CSCs). Arabidopsis thaliana GH9A1/KORRIGAN1 is a membrane-bound, family 9 glycosyl hydrolase that is important for cellulose synthesis in both primary and secondary cell walls. Most previously identified korrigan1 mutants show severe phenotypes such as embryo lethality; therefore, the role of GH9A1 in cellulose synthesis remains unclear. Here, we report a novel A577V missense mutation, designated jiaoyao1 (jia1), in the second of the glycosyl hydrolase family 9 active site signature motifs in GH9A1. jia1 is defective in cell expansion in dark-grown hypocotyls, roots, and adult plants. Consistent with its defect in cell expansion, this mutation in GH9A1 resulted in reduced cellulose content and reduced CSC velocity at the plasma membrane. Green fluorescent protein-GH9A1 is associated with CSCs at multiple locations, including the plasma membrane, Golgi, trans-Golgi network, and small CESA-containing compartments or microtubule-associated cellulose synthase compartments, indicating a tight association between GH9A1 and CSCs. GH9A1A577V abolishes the endoglucanase activity of GH9A1 in vitro but does not affect its interaction with CESAs in vitro, suggesting that endoglucanase activity is important for cellulose synthesis. Interestingly, jia1 results in both cellulose microfibril and microtubule disorganization. Our study establishes the important role of endoglucanase in cellulose synthesis and cellulose microfibril organization in plants.",
author = "Lei Lei and Tian Zhang and Richard Strasser and Lee, {Christopher M.} and Martine Gonneau and Lukas Mach and Samantha Vernhettes and Kim, {Seong H.} and Cosgrove, {Daniel J.} and Shundai Li and Ying Gu",
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The jiaoyao1 mutant is an allele of korrigan1 that abolishes endoglucanase activity and affects the organization of both cellulose microfibrils and microtubules in Arabidopsis. / Lei, Lei; Zhang, Tian; Strasser, Richard; Lee, Christopher M.; Gonneau, Martine; Mach, Lukas; Vernhettes, Samantha; Kim, Seong H.; Cosgrove, Daniel J.; Li, Shundai; Gu, Ying.

In: Plant Cell, Vol. 26, No. 6, 07.2014, p. 2601-2616.

Research output: Contribution to journalArticle

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T1 - The jiaoyao1 mutant is an allele of korrigan1 that abolishes endoglucanase activity and affects the organization of both cellulose microfibrils and microtubules in Arabidopsis

AU - Lei, Lei

AU - Zhang, Tian

AU - Strasser, Richard

AU - Lee, Christopher M.

AU - Gonneau, Martine

AU - Mach, Lukas

AU - Vernhettes, Samantha

AU - Kim, Seong H.

AU - Cosgrove, Daniel J.

AU - Li, Shundai

AU - Gu, Ying

PY - 2014/7

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N2 - In higher plants, cellulose is synthesized by plasma membrane-localized cellulose synthase complexes (CSCs). Arabidopsis thaliana GH9A1/KORRIGAN1 is a membrane-bound, family 9 glycosyl hydrolase that is important for cellulose synthesis in both primary and secondary cell walls. Most previously identified korrigan1 mutants show severe phenotypes such as embryo lethality; therefore, the role of GH9A1 in cellulose synthesis remains unclear. Here, we report a novel A577V missense mutation, designated jiaoyao1 (jia1), in the second of the glycosyl hydrolase family 9 active site signature motifs in GH9A1. jia1 is defective in cell expansion in dark-grown hypocotyls, roots, and adult plants. Consistent with its defect in cell expansion, this mutation in GH9A1 resulted in reduced cellulose content and reduced CSC velocity at the plasma membrane. Green fluorescent protein-GH9A1 is associated with CSCs at multiple locations, including the plasma membrane, Golgi, trans-Golgi network, and small CESA-containing compartments or microtubule-associated cellulose synthase compartments, indicating a tight association between GH9A1 and CSCs. GH9A1A577V abolishes the endoglucanase activity of GH9A1 in vitro but does not affect its interaction with CESAs in vitro, suggesting that endoglucanase activity is important for cellulose synthesis. Interestingly, jia1 results in both cellulose microfibril and microtubule disorganization. Our study establishes the important role of endoglucanase in cellulose synthesis and cellulose microfibril organization in plants.

AB - In higher plants, cellulose is synthesized by plasma membrane-localized cellulose synthase complexes (CSCs). Arabidopsis thaliana GH9A1/KORRIGAN1 is a membrane-bound, family 9 glycosyl hydrolase that is important for cellulose synthesis in both primary and secondary cell walls. Most previously identified korrigan1 mutants show severe phenotypes such as embryo lethality; therefore, the role of GH9A1 in cellulose synthesis remains unclear. Here, we report a novel A577V missense mutation, designated jiaoyao1 (jia1), in the second of the glycosyl hydrolase family 9 active site signature motifs in GH9A1. jia1 is defective in cell expansion in dark-grown hypocotyls, roots, and adult plants. Consistent with its defect in cell expansion, this mutation in GH9A1 resulted in reduced cellulose content and reduced CSC velocity at the plasma membrane. Green fluorescent protein-GH9A1 is associated with CSCs at multiple locations, including the plasma membrane, Golgi, trans-Golgi network, and small CESA-containing compartments or microtubule-associated cellulose synthase compartments, indicating a tight association between GH9A1 and CSCs. GH9A1A577V abolishes the endoglucanase activity of GH9A1 in vitro but does not affect its interaction with CESAs in vitro, suggesting that endoglucanase activity is important for cellulose synthesis. Interestingly, jia1 results in both cellulose microfibril and microtubule disorganization. Our study establishes the important role of endoglucanase in cellulose synthesis and cellulose microfibril organization in plants.

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