The kinetoplast duplication cycle in trypanosoma brucei is orchestrated by cytoskeleton-mediated cell morphogenesis

Eva Gluenz, Megan L. Povelones, Paul T. Englund, Keith Gull

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The mitochondrial DNA of Trypanosoma brucei is organized in a complex structure called the kinetoplast. In this study, we define the complete kinetoplast duplication cycle in T. brucei based on three-dimensional reconstructions from serial-section electron micrographs. This structural model was enhanced by analyses of the replication process of DNA maxi- and minicircles. Novel insights were obtained about the earliest and latest stages of kinetoplast duplication. We show that kinetoplast S phase occurs concurrently with the repositioning of the new basal body from the anterior to the posterior side of the old flagellum. This emphasizes the role of basal body segregation in kinetoplast division and suggests a possible mechanism for driving the rotational movement of the kinetoplast during minicircle replication. Fluorescence in situ hybridization with minicircle- and maxicircle-specific probes showed that maxicircle DNA is stretched out between segregated minicircle networks, indicating that maxicircle segregation is a late event in the kinetoplast duplication cycle. This new view of the complexities of kinetoplast duplication emphasizes the dependencies between the dynamic remodelling of the cytoskeleton and the inheritance of the mitochondrial genome.

Original languageEnglish (US)
Pages (from-to)1012-1021
Number of pages10
JournalMolecular and cellular biology
Volume31
Issue number5
DOIs
StatePublished - Mar 1 2011

Fingerprint

Basal Bodies
Trypanosoma brucei brucei
Cytoskeleton
Morphogenesis
Mitochondrial Genome
Flagella
Structural Models
Fluorescence In Situ Hybridization
DNA Replication
Mitochondrial DNA
S Phase
Electrons
DNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

@article{03f771ca25984f249ca69a27f7a76188,
title = "The kinetoplast duplication cycle in trypanosoma brucei is orchestrated by cytoskeleton-mediated cell morphogenesis",
abstract = "The mitochondrial DNA of Trypanosoma brucei is organized in a complex structure called the kinetoplast. In this study, we define the complete kinetoplast duplication cycle in T. brucei based on three-dimensional reconstructions from serial-section electron micrographs. This structural model was enhanced by analyses of the replication process of DNA maxi- and minicircles. Novel insights were obtained about the earliest and latest stages of kinetoplast duplication. We show that kinetoplast S phase occurs concurrently with the repositioning of the new basal body from the anterior to the posterior side of the old flagellum. This emphasizes the role of basal body segregation in kinetoplast division and suggests a possible mechanism for driving the rotational movement of the kinetoplast during minicircle replication. Fluorescence in situ hybridization with minicircle- and maxicircle-specific probes showed that maxicircle DNA is stretched out between segregated minicircle networks, indicating that maxicircle segregation is a late event in the kinetoplast duplication cycle. This new view of the complexities of kinetoplast duplication emphasizes the dependencies between the dynamic remodelling of the cytoskeleton and the inheritance of the mitochondrial genome.",
author = "Eva Gluenz and Povelones, {Megan L.} and Englund, {Paul T.} and Keith Gull",
year = "2011",
month = "3",
day = "1",
doi = "10.1128/MCB.01176-10",
language = "English (US)",
volume = "31",
pages = "1012--1021",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "5",

}

The kinetoplast duplication cycle in trypanosoma brucei is orchestrated by cytoskeleton-mediated cell morphogenesis. / Gluenz, Eva; Povelones, Megan L.; Englund, Paul T.; Gull, Keith.

In: Molecular and cellular biology, Vol. 31, No. 5, 01.03.2011, p. 1012-1021.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The kinetoplast duplication cycle in trypanosoma brucei is orchestrated by cytoskeleton-mediated cell morphogenesis

AU - Gluenz, Eva

AU - Povelones, Megan L.

AU - Englund, Paul T.

AU - Gull, Keith

PY - 2011/3/1

Y1 - 2011/3/1

N2 - The mitochondrial DNA of Trypanosoma brucei is organized in a complex structure called the kinetoplast. In this study, we define the complete kinetoplast duplication cycle in T. brucei based on three-dimensional reconstructions from serial-section electron micrographs. This structural model was enhanced by analyses of the replication process of DNA maxi- and minicircles. Novel insights were obtained about the earliest and latest stages of kinetoplast duplication. We show that kinetoplast S phase occurs concurrently with the repositioning of the new basal body from the anterior to the posterior side of the old flagellum. This emphasizes the role of basal body segregation in kinetoplast division and suggests a possible mechanism for driving the rotational movement of the kinetoplast during minicircle replication. Fluorescence in situ hybridization with minicircle- and maxicircle-specific probes showed that maxicircle DNA is stretched out between segregated minicircle networks, indicating that maxicircle segregation is a late event in the kinetoplast duplication cycle. This new view of the complexities of kinetoplast duplication emphasizes the dependencies between the dynamic remodelling of the cytoskeleton and the inheritance of the mitochondrial genome.

AB - The mitochondrial DNA of Trypanosoma brucei is organized in a complex structure called the kinetoplast. In this study, we define the complete kinetoplast duplication cycle in T. brucei based on three-dimensional reconstructions from serial-section electron micrographs. This structural model was enhanced by analyses of the replication process of DNA maxi- and minicircles. Novel insights were obtained about the earliest and latest stages of kinetoplast duplication. We show that kinetoplast S phase occurs concurrently with the repositioning of the new basal body from the anterior to the posterior side of the old flagellum. This emphasizes the role of basal body segregation in kinetoplast division and suggests a possible mechanism for driving the rotational movement of the kinetoplast during minicircle replication. Fluorescence in situ hybridization with minicircle- and maxicircle-specific probes showed that maxicircle DNA is stretched out between segregated minicircle networks, indicating that maxicircle segregation is a late event in the kinetoplast duplication cycle. This new view of the complexities of kinetoplast duplication emphasizes the dependencies between the dynamic remodelling of the cytoskeleton and the inheritance of the mitochondrial genome.

UR - http://www.scopus.com/inward/record.url?scp=79952273671&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952273671&partnerID=8YFLogxK

U2 - 10.1128/MCB.01176-10

DO - 10.1128/MCB.01176-10

M3 - Article

C2 - 21173163

AN - SCOPUS:79952273671

VL - 31

SP - 1012

EP - 1021

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 5

ER -