The L gene of J paramyxovirus plays a critical role in viral pathogenesis

Zhuo Li, Jie Xu, Zhenhai Chen, Xiudan Gao, Lin Fa Wang, Christopher Basler, Kaori Sakamoto, Biao He

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

J paramyxovirus (JPV) was first isolated from moribund mice with hemorrhagic lung lesions in Australia in the 1970s. Recent sequencing of JPV (JPV-LW) confirms that JPV is a paramyxovirus with several unique features. However, neither JPV-LW nor a recombinant JPV based on its sequence (rJPV-LW) caused obvious illness in mice. In this work, we analyzed a different JPV isolate (JPV-BH), which behaved differently from JPV-LW; JPV-BH grew more slowly in Vero cells and had less of a cytopathic effect on tissue culture cells but caused severe disease in mice. We have determined the whole genome sequence of JPV-BH. There were several nucleotide sequence differences between JPV-BH and JPV-LW, one in the leader sequence, one in the GX gene, and three in the L gene. The high sequence identity between JPV-BH and JPV-LW suggests that JPV-BH and JPV-LW are the same virus strain but were obtained at different passages from different laboratories. To understand the roles of these nucleotide sequence differences in pathogenicity in mice, we generated a recombinant JPV-BH strain (rJPV-BH) and hybrid rJPV-BH strains with sequences from the leader sequence (rJPV-BH-Le-LW), the GX gene (rJPV-BH-GX-LW), and the L gene (rJPV-BH-L-LW) of JPV-LW and compared their pathogenicities in mice. We have found that rJPV-BH-L-LW was attenuated in mice, indicating that nucleotide sequence differences in the L gene play a critical role in pathogenesis.

Original languageEnglish (US)
Pages (from-to)12990-12998
Number of pages9
JournalJournal of virology
Volume87
Issue number23
DOIs
StatePublished - Dec 1 2013

Fingerprint

Respirovirus
pathogenesis
Genes
genes
Virulence
Vero Cells
mice
Cell Culture Techniques
Genome
Viruses
Lung
nucleotide sequences
pathogenicity

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Li, Z., Xu, J., Chen, Z., Gao, X., Wang, L. F., Basler, C., ... He, B. (2013). The L gene of J paramyxovirus plays a critical role in viral pathogenesis. Journal of virology, 87(23), 12990-12998. https://doi.org/10.1128/JVI.02039-13
Li, Zhuo ; Xu, Jie ; Chen, Zhenhai ; Gao, Xiudan ; Wang, Lin Fa ; Basler, Christopher ; Sakamoto, Kaori ; He, Biao. / The L gene of J paramyxovirus plays a critical role in viral pathogenesis. In: Journal of virology. 2013 ; Vol. 87, No. 23. pp. 12990-12998.
@article{274ee6408bf94698952c1c6af2278066,
title = "The L gene of J paramyxovirus plays a critical role in viral pathogenesis",
abstract = "J paramyxovirus (JPV) was first isolated from moribund mice with hemorrhagic lung lesions in Australia in the 1970s. Recent sequencing of JPV (JPV-LW) confirms that JPV is a paramyxovirus with several unique features. However, neither JPV-LW nor a recombinant JPV based on its sequence (rJPV-LW) caused obvious illness in mice. In this work, we analyzed a different JPV isolate (JPV-BH), which behaved differently from JPV-LW; JPV-BH grew more slowly in Vero cells and had less of a cytopathic effect on tissue culture cells but caused severe disease in mice. We have determined the whole genome sequence of JPV-BH. There were several nucleotide sequence differences between JPV-BH and JPV-LW, one in the leader sequence, one in the GX gene, and three in the L gene. The high sequence identity between JPV-BH and JPV-LW suggests that JPV-BH and JPV-LW are the same virus strain but were obtained at different passages from different laboratories. To understand the roles of these nucleotide sequence differences in pathogenicity in mice, we generated a recombinant JPV-BH strain (rJPV-BH) and hybrid rJPV-BH strains with sequences from the leader sequence (rJPV-BH-Le-LW), the GX gene (rJPV-BH-GX-LW), and the L gene (rJPV-BH-L-LW) of JPV-LW and compared their pathogenicities in mice. We have found that rJPV-BH-L-LW was attenuated in mice, indicating that nucleotide sequence differences in the L gene play a critical role in pathogenesis.",
author = "Zhuo Li and Jie Xu and Zhenhai Chen and Xiudan Gao and Wang, {Lin Fa} and Christopher Basler and Kaori Sakamoto and Biao He",
year = "2013",
month = "12",
day = "1",
doi = "10.1128/JVI.02039-13",
language = "English (US)",
volume = "87",
pages = "12990--12998",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "23",

}

Li, Z, Xu, J, Chen, Z, Gao, X, Wang, LF, Basler, C, Sakamoto, K & He, B 2013, 'The L gene of J paramyxovirus plays a critical role in viral pathogenesis', Journal of virology, vol. 87, no. 23, pp. 12990-12998. https://doi.org/10.1128/JVI.02039-13

The L gene of J paramyxovirus plays a critical role in viral pathogenesis. / Li, Zhuo; Xu, Jie; Chen, Zhenhai; Gao, Xiudan; Wang, Lin Fa; Basler, Christopher; Sakamoto, Kaori; He, Biao.

In: Journal of virology, Vol. 87, No. 23, 01.12.2013, p. 12990-12998.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The L gene of J paramyxovirus plays a critical role in viral pathogenesis

AU - Li, Zhuo

AU - Xu, Jie

AU - Chen, Zhenhai

AU - Gao, Xiudan

AU - Wang, Lin Fa

AU - Basler, Christopher

AU - Sakamoto, Kaori

AU - He, Biao

PY - 2013/12/1

Y1 - 2013/12/1

N2 - J paramyxovirus (JPV) was first isolated from moribund mice with hemorrhagic lung lesions in Australia in the 1970s. Recent sequencing of JPV (JPV-LW) confirms that JPV is a paramyxovirus with several unique features. However, neither JPV-LW nor a recombinant JPV based on its sequence (rJPV-LW) caused obvious illness in mice. In this work, we analyzed a different JPV isolate (JPV-BH), which behaved differently from JPV-LW; JPV-BH grew more slowly in Vero cells and had less of a cytopathic effect on tissue culture cells but caused severe disease in mice. We have determined the whole genome sequence of JPV-BH. There were several nucleotide sequence differences between JPV-BH and JPV-LW, one in the leader sequence, one in the GX gene, and three in the L gene. The high sequence identity between JPV-BH and JPV-LW suggests that JPV-BH and JPV-LW are the same virus strain but were obtained at different passages from different laboratories. To understand the roles of these nucleotide sequence differences in pathogenicity in mice, we generated a recombinant JPV-BH strain (rJPV-BH) and hybrid rJPV-BH strains with sequences from the leader sequence (rJPV-BH-Le-LW), the GX gene (rJPV-BH-GX-LW), and the L gene (rJPV-BH-L-LW) of JPV-LW and compared their pathogenicities in mice. We have found that rJPV-BH-L-LW was attenuated in mice, indicating that nucleotide sequence differences in the L gene play a critical role in pathogenesis.

AB - J paramyxovirus (JPV) was first isolated from moribund mice with hemorrhagic lung lesions in Australia in the 1970s. Recent sequencing of JPV (JPV-LW) confirms that JPV is a paramyxovirus with several unique features. However, neither JPV-LW nor a recombinant JPV based on its sequence (rJPV-LW) caused obvious illness in mice. In this work, we analyzed a different JPV isolate (JPV-BH), which behaved differently from JPV-LW; JPV-BH grew more slowly in Vero cells and had less of a cytopathic effect on tissue culture cells but caused severe disease in mice. We have determined the whole genome sequence of JPV-BH. There were several nucleotide sequence differences between JPV-BH and JPV-LW, one in the leader sequence, one in the GX gene, and three in the L gene. The high sequence identity between JPV-BH and JPV-LW suggests that JPV-BH and JPV-LW are the same virus strain but were obtained at different passages from different laboratories. To understand the roles of these nucleotide sequence differences in pathogenicity in mice, we generated a recombinant JPV-BH strain (rJPV-BH) and hybrid rJPV-BH strains with sequences from the leader sequence (rJPV-BH-Le-LW), the GX gene (rJPV-BH-GX-LW), and the L gene (rJPV-BH-L-LW) of JPV-LW and compared their pathogenicities in mice. We have found that rJPV-BH-L-LW was attenuated in mice, indicating that nucleotide sequence differences in the L gene play a critical role in pathogenesis.

UR - http://www.scopus.com/inward/record.url?scp=84887202942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887202942&partnerID=8YFLogxK

U2 - 10.1128/JVI.02039-13

DO - 10.1128/JVI.02039-13

M3 - Article

C2 - 24067956

AN - SCOPUS:84887202942

VL - 87

SP - 12990

EP - 12998

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 23

ER -

Li Z, Xu J, Chen Z, Gao X, Wang LF, Basler C et al. The L gene of J paramyxovirus plays a critical role in viral pathogenesis. Journal of virology. 2013 Dec 1;87(23):12990-12998. https://doi.org/10.1128/JVI.02039-13