The maize EmBP-1 orthologue differentially regulates Opaque2-dependent gene expression in yeast and cultured maize endosperm cells

Leslie E. Carlini, Mariena Ketudat, Ronald L. Parsons, Shashidhar Prabhakar, Robert J. Schmidt, Mark J. Guiltinan

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In addition to the bZIP protein Opaque2 (O2), there are other maize endosperm nuclear proteins that recognize the O2 box in 22 kDa zein gene promoters. In an effort to understand the effect of these factors on 22 kDa zein expression, we have cloned one of these and identified it as the putative maize (Zea mays L.) orthologue of the wheat bZIP protein EmBP-1 (mEmBP-1). The mEmBP-1 protein exhibits 52% sequence identity and 68% similarity with the wheat protein and recognizes a similar spectrum of DNA sequences, albeit with slightly altered specificity. The mEmBP-1 gene exists as duplicate loci in maize on chromosomes 7S (mEmBP-1a) and 2L (mEmBP-1b). The mEmBP-1 genes are expressed in endosperm, embryo, immature ears, tassel, roots, and seedling shoots at low levels. Although mEmBP-1 binds to the 02 box from the 22 kDa zein gene promoter as a homodimer, it is unable to heterodimerize with O2. The mEmBP-1 protein can activate transcription from a truncated promoter containing a pentamer of the O2 site in yeast cells; however, it inhibited regulated transcription of a 22 kDa zein promoter in a transient expression assay using cultured maize endosperm cells.

Original languageEnglish (US)
Pages (from-to)339-349
Number of pages11
JournalPlant molecular biology
Volume41
Issue number3
DOIs
StatePublished - Oct 1 1999

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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