The mechanism of sphingosine enhancement of phorbol ester-mediated Phospholipase D activation in lymphocytes

Yu Zhang Cao, Andrea M. Mastro, Mary Lou Eskew, George Hildenbrandt, Padala V. Reddy, C. Channa Reddy

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Tumor-promoting phorbol esters stimulate the production of phosphatidylethanol (PEt) in bovine lymph node lymphocytes through the activation of Phospholipase D (PLD). Sphingosine enhanced the 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated formation of PEt in lymphocytes, while it had no effect by itself. Interestingly, the incorporation of labeled arachidonic acid into phospholipids was increased by sphingosine. Examination of the effect of sphingosine along with TPA on the incorporation of [1-14C]labeled-arachidonic acid into individual phospholipids revealed that they enhanced significantly the specific activity of only PC and lyse-PC fractions. These observations indicate that sphingosine does not activate PLD directly as TPA does. It appears that sphingosine increases the specific radioactivity of the substrate pool for PLD which results in an apparent increase in PEt when both sphingosine and TPA are present.

Original languageEnglish (US)
Pages (from-to)908-915
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume217
Issue number3
DOIs
StatePublished - Dec 26 1995

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The mechanism of sphingosine enhancement of phorbol ester-mediated Phospholipase D activation in lymphocytes'. Together they form a unique fingerprint.

  • Cite this