The mechanism of sphingosine enhancement of phorbol ester-mediated Phospholipase D activation in lymphocytes

Yu Zhang Cao, Andrea Marie Mastro, Mary Lou Eskew, George Hildenbrandt, Padala V. Reddy, C. Channa Reddy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tumor-promoting phorbol esters stimulate the production of phosphatidylethanol (PEt) in bovine lymph node lymphocytes through the activation of Phospholipase D (PLD). Sphingosine enhanced the 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated formation of PEt in lymphocytes, while it had no effect by itself. Interestingly, the incorporation of labeled arachidonic acid into phospholipids was increased by sphingosine. Examination of the effect of sphingosine along with TPA on the incorporation of [1- 14 C]labeled-arachidonic acid into individual phospholipids revealed that they enhanced significantly the specific activity of only PC and lyse-PC fractions. These observations indicate that sphingosine does not activate PLD directly as TPA does. It appears that sphingosine increases the specific radioactivity of the substrate pool for PLD which results in an apparent increase in PEt when both sphingosine and TPA are present.

Original languageEnglish (US)
Pages (from-to)908-915
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume217
Issue number3
DOIs
StatePublished - Dec 26 1995

Fingerprint

Phospholipase D
Sphingosine
Lymphocytes
Phorbol Esters
Lymphocyte Activation
Chemical activation
Tetradecanoylphorbol Acetate
Acetates
Arachidonic Acid
Phospholipids
Radioactivity
Tumors
Lymph Nodes
Substrates
phosphatidylethanol
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cao, Yu Zhang ; Mastro, Andrea Marie ; Eskew, Mary Lou ; Hildenbrandt, George ; Reddy, Padala V. ; Reddy, C. Channa. / The mechanism of sphingosine enhancement of phorbol ester-mediated Phospholipase D activation in lymphocytes. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 217, No. 3. pp. 908-915.
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The mechanism of sphingosine enhancement of phorbol ester-mediated Phospholipase D activation in lymphocytes. / Cao, Yu Zhang; Mastro, Andrea Marie; Eskew, Mary Lou; Hildenbrandt, George; Reddy, Padala V.; Reddy, C. Channa.

In: Biochemical and Biophysical Research Communications, Vol. 217, No. 3, 26.12.1995, p. 908-915.

Research output: Contribution to journalArticle

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