The menD and menE homologs code for 2-succinyl-6-hydroxyl-2,4-cyclohexadiene-1-carboxylate synthase and O-succinylbenzoic acid-CoA synthase in the phylloquinone biosynthetic pathway of Synechocystis sp. PCC 6803

T. Wade Johnson, Sushma Naithani, Charles Stewart, Boris Zybailov, A. Daniel Jones, John H. Golbeck, Parag R. Chitnis

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28 Scopus citations

Abstract

The genome of the cyanobacterium Synechocystis sp. PCC 6803 contains genes identified as menD and menE, homologs of Escherichia coli genes that code for 2-succinyl-6-hydroxyl-2,4-cyclohexadiene-1-carboxylate (SHCHC) synthase and O-succinylbenzoic acid-CoA ligase in the menaquinone biosynthetic pathway. In cyanobacteria, the product of this pathway is 2-methyl-3-phytyl-1,4-naphthoquinone (phylloquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem (PS) I. The menD- and menE- strains were generated, and both were found to lack phylloquinone. Hence, no alternative pathways exist in cyanobacteria to produce O-succinylbenzoyl-CoA. Q-band EPR studies of photoaccumulated quinone anion radical and optical kinetic studies of the P700+ [FA/FB]- backreaction indicate that in the mutant strains, plastoquinone-9 functions as the electron transfer cofactor in the A1 site of PS I. At a light intensity of 40 μE m-2 s-1, the menD- and menE- mutant strains grew photoautotrophically and photoheterotrophically, but with doubling times slower than the wild type. Both of which are sensitive to high light intensities. Low-temperature fluorescence studies show that in the menD- and menE- mutants, the ratio of PS I to PS II is reduced relative to the wild type. Whole-chain electron transfer rates in the menD- and menE- mutant cells are correspondingly higher on a chlorophyll basis. The slower growth rate and high-light sensitivity of the menD- and menE- mutants are therefore attributed to a lower content of PS I per cell.

Original languageEnglish (US)
Pages (from-to)67-76
Number of pages10
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1557
Issue number1-3
DOIs
StatePublished - Mar 6 2003

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

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