The microbiome of otitis media with effusion in Indigenous Australian children

Jake Jervis-Bardy, Geraint B. Rogers, Peter S. Morris, Heidi C. Smith-Vaughan, Elizabeth Nosworthy, Lex E.X. Leong, Renee J. Smith, Laura S. Weyrich, Jacques De Haan, A. Simon Carney, Amanda J. Leach, Stephen O'Leary, Robyn L. Marsh

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Introduction: Indigenous Australian children have a high prevalence of otitis media with effusion (OME) and associated conductive hearing loss. Only three microbiological studies of middle ear fluid (MEF) from Indigenous Australian children with OME have been reported. All of these were reliant on culture or species-specific PCR assays. The aim of this study was to characterise the middle ear fluid (MEF), adenoid and nasopharyngeal (NP) microbiomes of Indigenous Australian children, using culture-independent 16S rRNA gene sequencing. Methods: MEF, NP swabs and adenoid specimens were collected from 11 children in the Alice Springs region of Central Australia. Bacterial communities in these specimens were characterised using 16S rRNA gene sequencing. Results: The microbiota in MEF samples were dominated (>50% relative abundance) by operational taxonomic units (OTUs) consistent with Alloiococcus otitidis (6/11), Haemophilus influenzae (3/11) or Streptococcus sp. (specifically, Mitis group streptococci which includes Streptococcus pneumoniae) (1/11). Anatomical site selectivity was indicated by the presence of a single conserved Haemophilus OTU in 7/11 MEF samples. In comparison, there were ten distinct Haemophilus OTUs observed across the NP and adenoid samples. Despite significant differences between the MEF and NP/adenoid microbiomes, Streptococcus sp., H. influenzae and Moraxella catarrhalis OTUs were common to all sample types. Co-occurrence of classical otopathogens in paired MEF and NP/Adenoid samples is consistent with earlier culture-based studies. Conclusion: These data highlight the need to further assess H. influenzae traits important in otitis media and to understand the role of canal flora, especially A. otitidis, in populations with a high prevalence of tympanic membrane perforation.

Original languageEnglish (US)
Pages (from-to)1548-1555
Number of pages8
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume79
Issue number9
DOIs
StatePublished - Sep 1 2015

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Otitis Media with Effusion
Microbiota
Middle Ear
Adenoids
Haemophilus influenzae
Haemophilus
Streptococcus
rRNA Genes
Streptococcus mitis
Tympanic Membrane Perforation
Conductive Hearing Loss
Moraxella (Branhamella) catarrhalis
Otitis Media
Streptococcus pneumoniae
Polymerase Chain Reaction
Population

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Otorhinolaryngology

Cite this

Jervis-Bardy, J., Rogers, G. B., Morris, P. S., Smith-Vaughan, H. C., Nosworthy, E., Leong, L. E. X., ... Marsh, R. L. (2015). The microbiome of otitis media with effusion in Indigenous Australian children. International Journal of Pediatric Otorhinolaryngology, 79(9), 1548-1555. https://doi.org/10.1016/j.ijporl.2015.07.013
Jervis-Bardy, Jake ; Rogers, Geraint B. ; Morris, Peter S. ; Smith-Vaughan, Heidi C. ; Nosworthy, Elizabeth ; Leong, Lex E.X. ; Smith, Renee J. ; Weyrich, Laura S. ; De Haan, Jacques ; Carney, A. Simon ; Leach, Amanda J. ; O'Leary, Stephen ; Marsh, Robyn L. / The microbiome of otitis media with effusion in Indigenous Australian children. In: International Journal of Pediatric Otorhinolaryngology. 2015 ; Vol. 79, No. 9. pp. 1548-1555.
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abstract = "Introduction: Indigenous Australian children have a high prevalence of otitis media with effusion (OME) and associated conductive hearing loss. Only three microbiological studies of middle ear fluid (MEF) from Indigenous Australian children with OME have been reported. All of these were reliant on culture or species-specific PCR assays. The aim of this study was to characterise the middle ear fluid (MEF), adenoid and nasopharyngeal (NP) microbiomes of Indigenous Australian children, using culture-independent 16S rRNA gene sequencing. Methods: MEF, NP swabs and adenoid specimens were collected from 11 children in the Alice Springs region of Central Australia. Bacterial communities in these specimens were characterised using 16S rRNA gene sequencing. Results: The microbiota in MEF samples were dominated (>50{\%} relative abundance) by operational taxonomic units (OTUs) consistent with Alloiococcus otitidis (6/11), Haemophilus influenzae (3/11) or Streptococcus sp. (specifically, Mitis group streptococci which includes Streptococcus pneumoniae) (1/11). Anatomical site selectivity was indicated by the presence of a single conserved Haemophilus OTU in 7/11 MEF samples. In comparison, there were ten distinct Haemophilus OTUs observed across the NP and adenoid samples. Despite significant differences between the MEF and NP/adenoid microbiomes, Streptococcus sp., H. influenzae and Moraxella catarrhalis OTUs were common to all sample types. Co-occurrence of classical otopathogens in paired MEF and NP/Adenoid samples is consistent with earlier culture-based studies. Conclusion: These data highlight the need to further assess H. influenzae traits important in otitis media and to understand the role of canal flora, especially A. otitidis, in populations with a high prevalence of tympanic membrane perforation.",
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Jervis-Bardy, J, Rogers, GB, Morris, PS, Smith-Vaughan, HC, Nosworthy, E, Leong, LEX, Smith, RJ, Weyrich, LS, De Haan, J, Carney, AS, Leach, AJ, O'Leary, S & Marsh, RL 2015, 'The microbiome of otitis media with effusion in Indigenous Australian children', International Journal of Pediatric Otorhinolaryngology, vol. 79, no. 9, pp. 1548-1555. https://doi.org/10.1016/j.ijporl.2015.07.013

The microbiome of otitis media with effusion in Indigenous Australian children. / Jervis-Bardy, Jake; Rogers, Geraint B.; Morris, Peter S.; Smith-Vaughan, Heidi C.; Nosworthy, Elizabeth; Leong, Lex E.X.; Smith, Renee J.; Weyrich, Laura S.; De Haan, Jacques; Carney, A. Simon; Leach, Amanda J.; O'Leary, Stephen; Marsh, Robyn L.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 79, No. 9, 01.09.2015, p. 1548-1555.

Research output: Contribution to journalArticle

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AU - Jervis-Bardy, Jake

AU - Rogers, Geraint B.

AU - Morris, Peter S.

AU - Smith-Vaughan, Heidi C.

AU - Nosworthy, Elizabeth

AU - Leong, Lex E.X.

AU - Smith, Renee J.

AU - Weyrich, Laura S.

AU - De Haan, Jacques

AU - Carney, A. Simon

AU - Leach, Amanda J.

AU - O'Leary, Stephen

AU - Marsh, Robyn L.

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Jervis-Bardy J, Rogers GB, Morris PS, Smith-Vaughan HC, Nosworthy E, Leong LEX et al. The microbiome of otitis media with effusion in Indigenous Australian children. International Journal of Pediatric Otorhinolaryngology. 2015 Sep 1;79(9):1548-1555. https://doi.org/10.1016/j.ijporl.2015.07.013