TY - JOUR
T1 - The microbiomes of blowflies and houseflies as bacterial transmission reservoirs
AU - Junqueira, Ana Carolina M.
AU - Ratan, Aakrosh
AU - Acerbi, Enzo
AU - Drautz-Moses, Daniela I.
AU - Premkrishnan, Balakrishnan N.V.
AU - Costea, Paul I.
AU - Linz, Bodo
AU - Purbojati, Rikky W.
AU - Paulo, Daniel F.
AU - Gaultier, Nicolas E.
AU - Subramanian, Poorani
AU - Hasan, Nur A.
AU - Colwell, Rita R.
AU - Bork, Peer
AU - Azeredo-Espin, Ana Maria L.
AU - Bryant, Donald A.
AU - Schuster, Stephan C.
N1 - Funding Information:
The authors thank Rosangela Aparecida Rodrigues for technical assistance and sample processing, Gabriel Lopes Centoducatte for the help with sample collection, Tay Qi Xiang Martin and Sebastian Noel Wittekindt for 3D modelling of the fly. This project was funded by an institutional grant from Singapore Ministry of Education Academic Research Fund Tier 3 under the research grant MOE2013-T3–1–013, and the Singapore Centre for Environmental Life Sciences Engineering (SCELSE), whose research is supported by the National Research Foundation Singapore, Ministry of Education, Nanyang Technological University, and National University of Singapore, under its Research Centre of Excellence Program. Partial support was also provided by United Nations Joint FAO/IAEA missions to A.M.L.A.-E (IAEA: 14856/RO).
Publisher Copyright:
© 2017 The Author(s).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Blowflies and houseflies are mechanical vectors inhabiting synanthropic environments around the world. They feed and breed in fecal and decaying organic matter, but the microbiome they harbour and transport is largely uncharacterized. We sampled 116 individual houseflies and blowflies from varying habitats on three continents and subjected them to high-coverage, whole-genome shotgun sequencing. This allowed for genomic and metagenomic analyses of the host-associated microbiome at the species level. Both fly host species segregate based on principal coordinate analysis of their microbial communities, but they also show an overlapping core microbiome. Legs and wings displayed the largest microbial diversity and were shown to be an important route for microbial dispersion. The environmental sequencing approach presented here detected a stochastic distribution of human pathogens, such as Helicobacter pylori, thereby demonstrating the potential of flies as proxies for environmental and public health surveillance.
AB - Blowflies and houseflies are mechanical vectors inhabiting synanthropic environments around the world. They feed and breed in fecal and decaying organic matter, but the microbiome they harbour and transport is largely uncharacterized. We sampled 116 individual houseflies and blowflies from varying habitats on three continents and subjected them to high-coverage, whole-genome shotgun sequencing. This allowed for genomic and metagenomic analyses of the host-associated microbiome at the species level. Both fly host species segregate based on principal coordinate analysis of their microbial communities, but they also show an overlapping core microbiome. Legs and wings displayed the largest microbial diversity and were shown to be an important route for microbial dispersion. The environmental sequencing approach presented here detected a stochastic distribution of human pathogens, such as Helicobacter pylori, thereby demonstrating the potential of flies as proxies for environmental and public health surveillance.
UR - http://www.scopus.com/inward/record.url?scp=85035201974&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85035201974&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-16353-x
DO - 10.1038/s41598-017-16353-x
M3 - Article
C2 - 29176730
AN - SCOPUS:85035201974
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 16324
ER -
