The microbiomes of blowflies and houseflies as bacterial transmission reservoirs

Ana Carolina M. Junqueira, Aakrosh Ratan, Enzo Acerbi, Daniela I. Drautz-Moses, Balakrishnan N.V. Premkrishnan, Paul I. Costea, Bodo Linz, Rikky W. Purbojati, Daniel F. Paulo, Nicolas E. Gaultier, Poorani Subramanian, Nur A. Hasan, Rita R. Colwell, Peer Bork, Ana Maria L. Azeredo-Espin, Donald A. Bryant, Stephan C. Schuster

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Blowflies and houseflies are mechanical vectors inhabiting synanthropic environments around the world. They feed and breed in fecal and decaying organic matter, but the microbiome they harbour and transport is largely uncharacterized. We sampled 116 individual houseflies and blowflies from varying habitats on three continents and subjected them to high-coverage, whole-genome shotgun sequencing. This allowed for genomic and metagenomic analyses of the host-associated microbiome at the species level. Both fly host species segregate based on principal coordinate analysis of their microbial communities, but they also show an overlapping core microbiome. Legs and wings displayed the largest microbial diversity and were shown to be an important route for microbial dispersion. The environmental sequencing approach presented here detected a stochastic distribution of human pathogens, such as Helicobacter pylori, thereby demonstrating the potential of flies as proxies for environmental and public health surveillance.

Original languageEnglish (US)
Article number16353
JournalScientific reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

    Fingerprint

All Science Journal Classification (ASJC) codes

  • General

Cite this

Junqueira, A. C. M., Ratan, A., Acerbi, E., Drautz-Moses, D. I., Premkrishnan, B. N. V., Costea, P. I., Linz, B., Purbojati, R. W., Paulo, D. F., Gaultier, N. E., Subramanian, P., Hasan, N. A., Colwell, R. R., Bork, P., Azeredo-Espin, A. M. L., Bryant, D. A., & Schuster, S. C. (2017). The microbiomes of blowflies and houseflies as bacterial transmission reservoirs. Scientific reports, 7(1), [16353]. https://doi.org/10.1038/s41598-017-16353-x