The mimecan gene expressed in human pituitary and regulated by pituitary transcription factor-1 as a marker for diagnosing pituitary tumors

San Mei Hu, Feng Li, Hui Min Yu, Rong Ying Li, Qin Yun Ma, Ting Jun Ye, Zhen Yu Lu, Jia Lun Chen, Huai Dong Song

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Context: Mimecan, a secretory protein, belongs to a family of small leucine-rich proteoglycans (SLRPs). The physiological functions of mimecan have not been fully understood. Objective: We hypothesize that the mimecan gene expressed in the human pituitary and regulated by pituitary transcription factor-1 (Pit-1) might act as a marker for diagnosing pituitary tumors. Design: The clinical aspect of our work was a cross-sectional study. Setting and Patients: In total, 20 pituitary tumor samples were collected from January 1, 2002, to December 30, 2002, in Ruijin Hospital, Shanghai, China. Intervention: The number of pituitary tumors was limited. Collection of more pituitary tumor samples for additional observation will be necessary. Main Outcome Measures: The main outcomes were measured by Northern blot, in situ hybridization, immunohistochemical analysis, and so on. Results: The mimecan gene was expressed at a moderate level in the mouse pituitary gland by Northern blot analysis. Expression of mimecan mRNA and protein is also observed in the human anterior pituitary gland. Luciferase reporter analysis and electrophoretic mobility shift assays show that Pit-1 activates the human mimecan promoter through Pit-1 response element sites. In addition, our data also show that almost all the ACTH- or GH-positive pituitary tumors likely express mimecan protein, and only a portion of prolactin-, TSH-, FSH-, and LH-positive pituitary tumors express mimecan protein. Conclusions: This work provides insight into the regulating mechanism of mimecan in pituitary and suggests that mimecan may be an unidentified pituitary secretory protein, and certain pituitary cells secreting ACTH or GH also secrete mimecan.

Original languageEnglish (US)
Pages (from-to)6657-6664
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume90
Issue number12
DOIs
StatePublished - Dec 1 2005

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Pituitary Neoplasms
Tumors
Transcription Factors
Genes
Proteins
Northern Blotting
Adrenocorticotropic Hormone
Somatotrophs
Electrophoretic mobility
Anterior Pituitary Gland
Response Elements
Electrophoretic Mobility Shift Assay
Pituitary Gland
Proteoglycans
Luciferases
Leucine
Prolactin
In Situ Hybridization
Assays
China

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Hu, San Mei ; Li, Feng ; Yu, Hui Min ; Li, Rong Ying ; Ma, Qin Yun ; Ye, Ting Jun ; Lu, Zhen Yu ; Chen, Jia Lun ; Song, Huai Dong. / The mimecan gene expressed in human pituitary and regulated by pituitary transcription factor-1 as a marker for diagnosing pituitary tumors. In: Journal of Clinical Endocrinology and Metabolism. 2005 ; Vol. 90, No. 12. pp. 6657-6664.
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abstract = "Context: Mimecan, a secretory protein, belongs to a family of small leucine-rich proteoglycans (SLRPs). The physiological functions of mimecan have not been fully understood. Objective: We hypothesize that the mimecan gene expressed in the human pituitary and regulated by pituitary transcription factor-1 (Pit-1) might act as a marker for diagnosing pituitary tumors. Design: The clinical aspect of our work was a cross-sectional study. Setting and Patients: In total, 20 pituitary tumor samples were collected from January 1, 2002, to December 30, 2002, in Ruijin Hospital, Shanghai, China. Intervention: The number of pituitary tumors was limited. Collection of more pituitary tumor samples for additional observation will be necessary. Main Outcome Measures: The main outcomes were measured by Northern blot, in situ hybridization, immunohistochemical analysis, and so on. Results: The mimecan gene was expressed at a moderate level in the mouse pituitary gland by Northern blot analysis. Expression of mimecan mRNA and protein is also observed in the human anterior pituitary gland. Luciferase reporter analysis and electrophoretic mobility shift assays show that Pit-1 activates the human mimecan promoter through Pit-1 response element sites. In addition, our data also show that almost all the ACTH- or GH-positive pituitary tumors likely express mimecan protein, and only a portion of prolactin-, TSH-, FSH-, and LH-positive pituitary tumors express mimecan protein. Conclusions: This work provides insight into the regulating mechanism of mimecan in pituitary and suggests that mimecan may be an unidentified pituitary secretory protein, and certain pituitary cells secreting ACTH or GH also secrete mimecan.",
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The mimecan gene expressed in human pituitary and regulated by pituitary transcription factor-1 as a marker for diagnosing pituitary tumors. / Hu, San Mei; Li, Feng; Yu, Hui Min; Li, Rong Ying; Ma, Qin Yun; Ye, Ting Jun; Lu, Zhen Yu; Chen, Jia Lun; Song, Huai Dong.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 90, No. 12, 01.12.2005, p. 6657-6664.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The mimecan gene expressed in human pituitary and regulated by pituitary transcription factor-1 as a marker for diagnosing pituitary tumors

AU - Hu, San Mei

AU - Li, Feng

AU - Yu, Hui Min

AU - Li, Rong Ying

AU - Ma, Qin Yun

AU - Ye, Ting Jun

AU - Lu, Zhen Yu

AU - Chen, Jia Lun

AU - Song, Huai Dong

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Y1 - 2005/12/1

N2 - Context: Mimecan, a secretory protein, belongs to a family of small leucine-rich proteoglycans (SLRPs). The physiological functions of mimecan have not been fully understood. Objective: We hypothesize that the mimecan gene expressed in the human pituitary and regulated by pituitary transcription factor-1 (Pit-1) might act as a marker for diagnosing pituitary tumors. Design: The clinical aspect of our work was a cross-sectional study. Setting and Patients: In total, 20 pituitary tumor samples were collected from January 1, 2002, to December 30, 2002, in Ruijin Hospital, Shanghai, China. Intervention: The number of pituitary tumors was limited. Collection of more pituitary tumor samples for additional observation will be necessary. Main Outcome Measures: The main outcomes were measured by Northern blot, in situ hybridization, immunohistochemical analysis, and so on. Results: The mimecan gene was expressed at a moderate level in the mouse pituitary gland by Northern blot analysis. Expression of mimecan mRNA and protein is also observed in the human anterior pituitary gland. Luciferase reporter analysis and electrophoretic mobility shift assays show that Pit-1 activates the human mimecan promoter through Pit-1 response element sites. In addition, our data also show that almost all the ACTH- or GH-positive pituitary tumors likely express mimecan protein, and only a portion of prolactin-, TSH-, FSH-, and LH-positive pituitary tumors express mimecan protein. Conclusions: This work provides insight into the regulating mechanism of mimecan in pituitary and suggests that mimecan may be an unidentified pituitary secretory protein, and certain pituitary cells secreting ACTH or GH also secrete mimecan.

AB - Context: Mimecan, a secretory protein, belongs to a family of small leucine-rich proteoglycans (SLRPs). The physiological functions of mimecan have not been fully understood. Objective: We hypothesize that the mimecan gene expressed in the human pituitary and regulated by pituitary transcription factor-1 (Pit-1) might act as a marker for diagnosing pituitary tumors. Design: The clinical aspect of our work was a cross-sectional study. Setting and Patients: In total, 20 pituitary tumor samples were collected from January 1, 2002, to December 30, 2002, in Ruijin Hospital, Shanghai, China. Intervention: The number of pituitary tumors was limited. Collection of more pituitary tumor samples for additional observation will be necessary. Main Outcome Measures: The main outcomes were measured by Northern blot, in situ hybridization, immunohistochemical analysis, and so on. Results: The mimecan gene was expressed at a moderate level in the mouse pituitary gland by Northern blot analysis. Expression of mimecan mRNA and protein is also observed in the human anterior pituitary gland. Luciferase reporter analysis and electrophoretic mobility shift assays show that Pit-1 activates the human mimecan promoter through Pit-1 response element sites. In addition, our data also show that almost all the ACTH- or GH-positive pituitary tumors likely express mimecan protein, and only a portion of prolactin-, TSH-, FSH-, and LH-positive pituitary tumors express mimecan protein. Conclusions: This work provides insight into the regulating mechanism of mimecan in pituitary and suggests that mimecan may be an unidentified pituitary secretory protein, and certain pituitary cells secreting ACTH or GH also secrete mimecan.

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