The mouse-primed lymphocyte typing test. III. Dissociation of T lymphocyte-stimulating determinants and antibody-defined specificities of the I region-associated Ia antigens

A. B. Peck, J. Klein, H. Wigzell

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The mouse-primed lymphocyte typing (mPLT) assay, based on the sequential reactivation of specific immunocompetent alloantigen-reactive T blast cells in secondary mixed leukocyte culture (MLC) has been utilized to define the major histocompatibility complex (MHC) I region, or Class II-associated lymphocyte-stimulating (LS) determinants. The test panel of secondary stimulating cells has been expanded to include 28 B10.W lines (mouse strains carrying MHC regions derived from wild mice); thus, nearly 40 'independently derived' MHC haplotypes are now represented. Data obtained by using mPLT cells generated in primary MLC between I-AB or I-EC subregion disparate strain combinations reveal that 1) an absolute correlation between expression of the serologically defined Ia specificities and capacity to induce subsequent secondary MLC does not exist; 2) based on the apparent genetic derivations of the I-AB and I-EC subregions, T lymphocytes most likely recognize either the β-chain per se or an interaction product of the α-plus β-chains; and 3) multiple restimulations of mPLT cells with cells expressing cross-reactive serologically defined Ia specificities fail to select for cells with increased reactivity against shared specificities. Based on these observations, we conclude that T and B lymphocytes recognize different antigenic moieties expressed on the MHC Class II antigens.

Original languageEnglish (US)
Pages (from-to)1078-1086
Number of pages9
JournalJournal of Immunology
Volume125
Issue number3
StatePublished - Jan 1 1980

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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