The mouse Sb1.8 gene located at the distal end of the X chromosome is subject to X inactivation

Razla Sultana, David A. Adler, Susanne Edelhoff, Laura Carrel, Kwang Ho Lee, Verne C. Chapman, Huntington F. Willard, Christine M. Disteche

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The mouse homolog of the human DXS423E (SB1.8) gene has been isolated by screening a mouse cDNA library. Like its human counterpart, the mouse Sb1.8 gene is X-linked, as shown by Southern blot analysis and by in situ hybridization to metaphase chromosomes. Sb1.8 was sublocalized to band F of the mouse X chromosome, distal to Alas2 and proximal to DXPas1, which confirms a region of conservation between band Xp11.21-p11.22 in human and band XF in mouse. In situ hybridization also showed that the Smcx (Xe169) gene maps near Sb1.8 in band F. The Sb1.8 gene was shown to be highly conserved in mammals; partial DNA sequence analysis indicates 92% identity between the mouse and human genes. In contrast to the human DXS423E gene, the mouse Sb1.8 gene is subject to X Inactivation, as shown by restriction enzyme and sequence analysis of mRNA from mice with Searle's translocation (T(X; 16)16H). Absence of Sb1.8 expression from the inactive mouse X chromosome in vitro was confirmed by analysis of a cell line (Hobmski) in which the M.spretus X chromosome is inactivated. The Sb1.8 gene is a new member of a group of genes that escape X inactivation in human, but are inactivated in mouse.

Original languageEnglish (US)
Pages (from-to)257-263
Number of pages7
JournalHuman Molecular Genetics
Volume4
Issue number2
DOIs
StatePublished - Feb 1 1995

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X Chromosome Inactivation
X Chromosome
Genes
In Situ Hybridization
X-Linked Genes
Restriction Mapping
Metaphase
Southern Blotting
Gene Library
DNA Sequence Analysis
Sequence Analysis
Mammals
Chromosomes
Cell Line

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Sultana, R., Adler, D. A., Edelhoff, S., Carrel, L., Lee, K. H., Chapman, V. C., ... Disteche, C. M. (1995). The mouse Sb1.8 gene located at the distal end of the X chromosome is subject to X inactivation. Human Molecular Genetics, 4(2), 257-263. https://doi.org/10.1093/hmg/4.2.257
Sultana, Razla ; Adler, David A. ; Edelhoff, Susanne ; Carrel, Laura ; Lee, Kwang Ho ; Chapman, Verne C. ; Willard, Huntington F. ; Disteche, Christine M. / The mouse Sb1.8 gene located at the distal end of the X chromosome is subject to X inactivation. In: Human Molecular Genetics. 1995 ; Vol. 4, No. 2. pp. 257-263.
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abstract = "The mouse homolog of the human DXS423E (SB1.8) gene has been isolated by screening a mouse cDNA library. Like its human counterpart, the mouse Sb1.8 gene is X-linked, as shown by Southern blot analysis and by in situ hybridization to metaphase chromosomes. Sb1.8 was sublocalized to band F of the mouse X chromosome, distal to Alas2 and proximal to DXPas1, which confirms a region of conservation between band Xp11.21-p11.22 in human and band XF in mouse. In situ hybridization also showed that the Smcx (Xe169) gene maps near Sb1.8 in band F. The Sb1.8 gene was shown to be highly conserved in mammals; partial DNA sequence analysis indicates 92{\%} identity between the mouse and human genes. In contrast to the human DXS423E gene, the mouse Sb1.8 gene is subject to X Inactivation, as shown by restriction enzyme and sequence analysis of mRNA from mice with Searle's translocation (T(X; 16)16H). Absence of Sb1.8 expression from the inactive mouse X chromosome in vitro was confirmed by analysis of a cell line (Hobmski) in which the M.spretus X chromosome is inactivated. The Sb1.8 gene is a new member of a group of genes that escape X inactivation in human, but are inactivated in mouse.",
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Sultana, R, Adler, DA, Edelhoff, S, Carrel, L, Lee, KH, Chapman, VC, Willard, HF & Disteche, CM 1995, 'The mouse Sb1.8 gene located at the distal end of the X chromosome is subject to X inactivation', Human Molecular Genetics, vol. 4, no. 2, pp. 257-263. https://doi.org/10.1093/hmg/4.2.257

The mouse Sb1.8 gene located at the distal end of the X chromosome is subject to X inactivation. / Sultana, Razla; Adler, David A.; Edelhoff, Susanne; Carrel, Laura; Lee, Kwang Ho; Chapman, Verne C.; Willard, Huntington F.; Disteche, Christine M.

In: Human Molecular Genetics, Vol. 4, No. 2, 01.02.1995, p. 257-263.

Research output: Contribution to journalArticle

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T1 - The mouse Sb1.8 gene located at the distal end of the X chromosome is subject to X inactivation

AU - Sultana, Razla

AU - Adler, David A.

AU - Edelhoff, Susanne

AU - Carrel, Laura

AU - Lee, Kwang Ho

AU - Chapman, Verne C.

AU - Willard, Huntington F.

AU - Disteche, Christine M.

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