The multiple effects of ethylenediaminetetraacetate in several model lipid peroxidation systems

Ming Tien, Lee A. Morehouse, John R. Bucher, Steven D. Aust

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Experiments were performed which illustrate the various ways EDTA can influence lipid peroxidation. Either detergent-dispersed linoleate, or liposomes made from extracted microsomal phospholipids were utilized as substrates for peroxidation. Peroxidation was accomplished using Fe2+ or Fe3+. In systems utilizing Fe2+, EDTA chelation facilitated Fe2+ autoxidation which in turn caused peroxidation of detergent-dispersed linoleate. Peroxidation was not initiated during EDTA-Fe2+ autoxidation when the substrate lipids were in a liposomal configuration. Systems utilizing Fe3+ required an enzyme (either xanthine oxidase or NADPH-cytochrome P450 reductase) to reduce the iron for peroxidative activity. EDTA chelation of Fe3+ enhanced the xanthine oxidase and NADPH-cytochrome P450 reductase-catalyzed peroxidation of detergent-dispersed linoleate, presumably by facilitating the reduction of Fe3+. Catalase and mannitol inhibited both EDTA-Fe2+- and EDTA-Fe3+-dependent lipid peroxidation. EDTA-Fe3+ was not capable of initiating peroxidation of phospholipid liposomes following enzymatic reduction by either enzyme, but ADP-chelated iron effectively initiated liposomal peroxidation in similar systems. With xanthine oxidase-catalyzed peroxidation of liposomes with ADP-Fe3+, the inclusion of EDTA-Fe3+ caused a modest enhancement of activity. EDTA-Fe3+ greatly stimulated NADPH-cytochrome P450 reductase-catalyzed peroxidation of liposomes with ADP-Fe3+. In contrast, the addition of EDTA, rather than EDTA-Fe3+ inhibited the liposomal peroxidation catalyzed by either enzyme with ADP-Fe3+ when the EDTA concentration exceeded the concentration of Fe3+.

Original languageEnglish (US)
Pages (from-to)450-458
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume218
Issue number2
DOIs
StatePublished - Oct 15 1982

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Edetic Acid
Lipid Peroxidation
Lipids
Liposomes
NADPH-Ferrihemoprotein Reductase
Adenosine Diphosphate
Xanthine Oxidase
Linoleic Acid
Detergents
Chelation
Phospholipids
Enzymes
Iron
Mannitol
Substrates
Catalase

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Tien, Ming ; Morehouse, Lee A. ; Bucher, John R. ; Aust, Steven D. / The multiple effects of ethylenediaminetetraacetate in several model lipid peroxidation systems. In: Archives of Biochemistry and Biophysics. 1982 ; Vol. 218, No. 2. pp. 450-458.
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The multiple effects of ethylenediaminetetraacetate in several model lipid peroxidation systems. / Tien, Ming; Morehouse, Lee A.; Bucher, John R.; Aust, Steven D.

In: Archives of Biochemistry and Biophysics, Vol. 218, No. 2, 15.10.1982, p. 450-458.

Research output: Contribution to journalArticle

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