The N-acetyl-d-glucosamine repressor NagC of Vibrio fischeri facilitates colonization of Euprymna scolopes

Tim Miyashiro, Will Klein, Dane Oehlert, Xiaodan Cao, Julia Schwartzman, Edward G. Ruby

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

To successfully colonize and persist within a host niche, bacteria must properly regulate their gene expression profiles. The marine bacterium Vibrio fischeri establishes a mutualistic symbiosis within the light organ of the Hawaiian squid, Euprymna scolopes. Here, we show that the repressor NagC of V.fischeri directly regulates several chitin- and N-acetyl-d-glucosamine-utilization genes that are co-regulated during productive symbiosis. We also demonstrate that repression by NagC is relieved in the presence of N-acetyl-d-glucosamine-6-phosphate, the intracellular form of N-acetyl-d-glucosamine. We find that gene repression by NagC is critical for efficient colonization of E.scolopes. Further, our study shows that NagC regulates genes that affect the normal dynamics of host colonization.

Original languageEnglish (US)
Pages (from-to)894-903
Number of pages10
JournalMolecular Microbiology
Volume82
Issue number4
DOIs
StatePublished - Nov 1 2011

Fingerprint

Aliivibrio fischeri
Glucosamine
Symbiosis
Genes
Bacteria
Decapodiformes
Chitin
Transcriptome
Light

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Miyashiro, Tim ; Klein, Will ; Oehlert, Dane ; Cao, Xiaodan ; Schwartzman, Julia ; Ruby, Edward G. / The N-acetyl-d-glucosamine repressor NagC of Vibrio fischeri facilitates colonization of Euprymna scolopes. In: Molecular Microbiology. 2011 ; Vol. 82, No. 4. pp. 894-903.
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The N-acetyl-d-glucosamine repressor NagC of Vibrio fischeri facilitates colonization of Euprymna scolopes. / Miyashiro, Tim; Klein, Will; Oehlert, Dane; Cao, Xiaodan; Schwartzman, Julia; Ruby, Edward G.

In: Molecular Microbiology, Vol. 82, No. 4, 01.11.2011, p. 894-903.

Research output: Contribution to journalArticle

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