The occludin and ZO-1 complex, defined by small angle X-ray scattering and NMR, has implications for modulating tight junction permeability

Brian R. Tash, Maria C. Bewley, Mariano Russo, Jason M. Keil, Kathleen A. Griffin, Jeffrey M. Sundstrom, David A. Antonetti, Fang Tian, John M. Flanagan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Tight junctions (TJs) are dynamic cellular structures that are critical for compartmentalizing environments within tissues and regulating transport of small molecules, ions, and fluids. Phosphorylation-dependent binding of the transmembrane protein occludin to the structural organizing protein ZO-1 contributes to the regulation of barrier properties; however, the details of their interaction are controversial. Using small angle X-ray scattering (SAXS), NMR chemical shift perturbation, cross-saturation, in vitro binding, and site-directed mutagenesis experiments. we define the interface between the ZO-1 PDZ3-SH3-U5-GuK (PSG) and occludin coiled-coil (CC) domains. The interface is comprised of basic residues in PSG and an acidic region in CC. Complex formation is blocked by a peptide (REESEEYM) that corresponds to CC residues 468-475 and includes a previously uncharacterized phosphosite, with the phosphorylated version having a larger effect. Furthermore, mutation of E470 and E472 reduces cell border localization of occludin. Together, these results localize the interaction to an acidic region in CC and a predominantly basic helix V within the ZO-1 GuK domain. This model has important implications for the phosphorylation-dependent regulation of the occludin:ZO-1 complex.

Original languageEnglish (US)
Pages (from-to)10855-10860
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number27
DOIs
StatePublished - Jul 3 2012

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Occludin
Tight Junctions
Permeability
X-Rays
Phosphorylation
Cellular Structures
Site-Directed Mutagenesis
Carrier Proteins
Binding Sites
Ions
Peptides
Mutation
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "The occludin and ZO-1 complex, defined by small angle X-ray scattering and NMR, has implications for modulating tight junction permeability",
abstract = "Tight junctions (TJs) are dynamic cellular structures that are critical for compartmentalizing environments within tissues and regulating transport of small molecules, ions, and fluids. Phosphorylation-dependent binding of the transmembrane protein occludin to the structural organizing protein ZO-1 contributes to the regulation of barrier properties; however, the details of their interaction are controversial. Using small angle X-ray scattering (SAXS), NMR chemical shift perturbation, cross-saturation, in vitro binding, and site-directed mutagenesis experiments. we define the interface between the ZO-1 PDZ3-SH3-U5-GuK (PSG) and occludin coiled-coil (CC) domains. The interface is comprised of basic residues in PSG and an acidic region in CC. Complex formation is blocked by a peptide (REESEEYM) that corresponds to CC residues 468-475 and includes a previously uncharacterized phosphosite, with the phosphorylated version having a larger effect. Furthermore, mutation of E470 and E472 reduces cell border localization of occludin. Together, these results localize the interaction to an acidic region in CC and a predominantly basic helix V within the ZO-1 GuK domain. This model has important implications for the phosphorylation-dependent regulation of the occludin:ZO-1 complex.",
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The occludin and ZO-1 complex, defined by small angle X-ray scattering and NMR, has implications for modulating tight junction permeability. / Tash, Brian R.; Bewley, Maria C.; Russo, Mariano; Keil, Jason M.; Griffin, Kathleen A.; Sundstrom, Jeffrey M.; Antonetti, David A.; Tian, Fang; Flanagan, John M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 27, 03.07.2012, p. 10855-10860.

Research output: Contribution to journalArticle

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T1 - The occludin and ZO-1 complex, defined by small angle X-ray scattering and NMR, has implications for modulating tight junction permeability

AU - Tash, Brian R.

AU - Bewley, Maria C.

AU - Russo, Mariano

AU - Keil, Jason M.

AU - Griffin, Kathleen A.

AU - Sundstrom, Jeffrey M.

AU - Antonetti, David A.

AU - Tian, Fang

AU - Flanagan, John M.

PY - 2012/7/3

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