The occurrence of multiple forms of cytochrome P-450 in hepatic microsomes from untreated rats and mice

Richard Mailman, Laurence G. Tate, Kenneth E. Muse, Lewis B. Coons, Ernest Hodgson

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The hepatic microsomes of rat and mice were subfractionated by the procedure of Dallner. When a 1.3 M sucrose lower layer was used for the two-step discontinuous gradient, no differences in spectral characteristics were noted between subfractions, though the smooth fractions (SER) had higher oxidative activity towards the substrates tested. When lower layers of 1.05, 1.1 or 1.15 M sucrose were used, the SER isolated contained cytochrome P-450 with significantly different spectral characteristics from that of the rough fraction (RER). The SER cytochrome P-450 had a wavelength maximum in the carbon-monoxide reduced difference spectrum that was significantly lower (ca. 1.0 nm) than that in the RER. In addition, the type I:CO-reduced spectral ratio of these fractions is significantly elevated. These data indicate that liver microsomes from untreated rats and mice contain more than one cytochrome P-450 and that these cytochromes may be located in different parts of the endoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)215-228
Number of pages14
JournalChemico-Biological Interactions
Volume10
Issue number3
DOIs
StatePublished - Jan 1 1975

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Microsomes
Cytochrome P-450 Enzyme System
Rats
Carbon Monoxide
Sucrose
Liver
Liver Microsomes
Cytochromes
Endoplasmic Reticulum
Wavelength
Substrates

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Mailman, Richard ; Tate, Laurence G. ; Muse, Kenneth E. ; Coons, Lewis B. ; Hodgson, Ernest. / The occurrence of multiple forms of cytochrome P-450 in hepatic microsomes from untreated rats and mice. In: Chemico-Biological Interactions. 1975 ; Vol. 10, No. 3. pp. 215-228.
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The occurrence of multiple forms of cytochrome P-450 in hepatic microsomes from untreated rats and mice. / Mailman, Richard; Tate, Laurence G.; Muse, Kenneth E.; Coons, Lewis B.; Hodgson, Ernest.

In: Chemico-Biological Interactions, Vol. 10, No. 3, 01.01.1975, p. 215-228.

Research output: Contribution to journalArticle

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