The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights

Jessica R. McCann, Nathan A. Bihlmeyer, Kimberly Roche, Cameron Catherine, Jayanth Jawahar, Lydia Coulter Kwee, Noelle E. Younge, Justin Silverman, Olga Ilkayeva, Charles Sarria, Alexandra Zizzi, Janet Wootton, Lisa Poppe, Paul Anderson, Michelle Arlotto, Zhengzheng Wei, Joshua A. Granek, Raphael H. Valdivia, Lawrence A. David, Holly K. DressmanChristopher B. Newgard, Svati H. Shah, Patrick C. Seed, John F. Rawls, Sarah C. Armstrong

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Objective: The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification. Methods: A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis—including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)—are presented. Results: Adolescents with obesity have higher serum alanine aminotransferase, C-reactive protein, and glycated hemoglobin, and they have lower high-density lipoprotein cholesterol when compared with healthy weight controls. Metabolomics revealed differences in branched-chain amino acid–related metabolites. Also observed was a differential abundance of specific microbial taxa and lower species diversity among adolescents with obesity when compared with the healthy weight group. Conclusions: The Pediatric Metabolism and Microbiome Study (POMMS) biorepository is available as a shared resource. Early findings suggest evidence of a metabolic signature of obesity unique to adolescents, along with confirmation of previously reported findings that describe metabolic and microbiome markers of obesity.

    Original languageEnglish (US)
    Pages (from-to)569-578
    Number of pages10
    JournalObesity
    Volume29
    Issue number3
    DOIs
    StatePublished - Mar 2021

    All Science Journal Classification (ASJC) codes

    • Medicine (miscellaneous)
    • Endocrinology, Diabetes and Metabolism
    • Endocrinology
    • Nutrition and Dietetics

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