The phantom burster model for pancreatic β-cells

Richard Bertram, Joseph Previte, Arthur Sherman, Tracie A. Kinard, Leslie S. Satin

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Pancreatic β-cells exhibit bursting oscillations with a wide range of periods. Whereas periods in isolated cells are generally either a few seconds or a few minutes, in intact islets of Langerhans they are intermediate (10-60 s). We develop a mathematical model for β-cell electrical activity capable of generating this wide range of bursting oscillations. Unlike previous models, bursting is driven by the interaction of two slow processes, one with a relatively small time constant (1-5 s) and the other with a much larger time constant (1-2 min). Bursting on the intermediate time scale is generated without need for a slow process having an intermediate time constant, hence phantom bursting. The model suggests that isolated cells exhibiting a fast pattern may nonetheless possess slower processes that can be brought out by injecting suitable exogenous currents. Guided by this, we devise an experimental protocol using the dynamic clamp technique that reliably elicits islet-like, medium period oscillations from isolated cells. Finally, we show that strong electrical coupling between a fast burster and a slow burster can produce synchronized medium bursting, suggesting that islets may be composed of cells that are intrinsically either fast or slow, with few or none that are intrinsically medium.

Original languageEnglish (US)
Pages (from-to)2880-2892
Number of pages13
JournalBiophysical journal
Volume79
Issue number6
DOIs
StatePublished - Dec 2000

Fingerprint

Islets of Langerhans
Theoretical Models

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

Bertram, R., Previte, J., Sherman, A., Kinard, T. A., & Satin, L. S. (2000). The phantom burster model for pancreatic β-cells. Biophysical journal, 79(6), 2880-2892. https://doi.org/10.1016/S0006-3495(00)76525-8
Bertram, Richard ; Previte, Joseph ; Sherman, Arthur ; Kinard, Tracie A. ; Satin, Leslie S. / The phantom burster model for pancreatic β-cells. In: Biophysical journal. 2000 ; Vol. 79, No. 6. pp. 2880-2892.
@article{ade2eb1021594ca699afb52ab4a9b354,
title = "The phantom burster model for pancreatic β-cells",
abstract = "Pancreatic β-cells exhibit bursting oscillations with a wide range of periods. Whereas periods in isolated cells are generally either a few seconds or a few minutes, in intact islets of Langerhans they are intermediate (10-60 s). We develop a mathematical model for β-cell electrical activity capable of generating this wide range of bursting oscillations. Unlike previous models, bursting is driven by the interaction of two slow processes, one with a relatively small time constant (1-5 s) and the other with a much larger time constant (1-2 min). Bursting on the intermediate time scale is generated without need for a slow process having an intermediate time constant, hence phantom bursting. The model suggests that isolated cells exhibiting a fast pattern may nonetheless possess slower processes that can be brought out by injecting suitable exogenous currents. Guided by this, we devise an experimental protocol using the dynamic clamp technique that reliably elicits islet-like, medium period oscillations from isolated cells. Finally, we show that strong electrical coupling between a fast burster and a slow burster can produce synchronized medium bursting, suggesting that islets may be composed of cells that are intrinsically either fast or slow, with few or none that are intrinsically medium.",
author = "Richard Bertram and Joseph Previte and Arthur Sherman and Kinard, {Tracie A.} and Satin, {Leslie S.}",
year = "2000",
month = "12",
doi = "10.1016/S0006-3495(00)76525-8",
language = "English (US)",
volume = "79",
pages = "2880--2892",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "6",

}

Bertram, R, Previte, J, Sherman, A, Kinard, TA & Satin, LS 2000, 'The phantom burster model for pancreatic β-cells', Biophysical journal, vol. 79, no. 6, pp. 2880-2892. https://doi.org/10.1016/S0006-3495(00)76525-8

The phantom burster model for pancreatic β-cells. / Bertram, Richard; Previte, Joseph; Sherman, Arthur; Kinard, Tracie A.; Satin, Leslie S.

In: Biophysical journal, Vol. 79, No. 6, 12.2000, p. 2880-2892.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The phantom burster model for pancreatic β-cells

AU - Bertram, Richard

AU - Previte, Joseph

AU - Sherman, Arthur

AU - Kinard, Tracie A.

AU - Satin, Leslie S.

PY - 2000/12

Y1 - 2000/12

N2 - Pancreatic β-cells exhibit bursting oscillations with a wide range of periods. Whereas periods in isolated cells are generally either a few seconds or a few minutes, in intact islets of Langerhans they are intermediate (10-60 s). We develop a mathematical model for β-cell electrical activity capable of generating this wide range of bursting oscillations. Unlike previous models, bursting is driven by the interaction of two slow processes, one with a relatively small time constant (1-5 s) and the other with a much larger time constant (1-2 min). Bursting on the intermediate time scale is generated without need for a slow process having an intermediate time constant, hence phantom bursting. The model suggests that isolated cells exhibiting a fast pattern may nonetheless possess slower processes that can be brought out by injecting suitable exogenous currents. Guided by this, we devise an experimental protocol using the dynamic clamp technique that reliably elicits islet-like, medium period oscillations from isolated cells. Finally, we show that strong electrical coupling between a fast burster and a slow burster can produce synchronized medium bursting, suggesting that islets may be composed of cells that are intrinsically either fast or slow, with few or none that are intrinsically medium.

AB - Pancreatic β-cells exhibit bursting oscillations with a wide range of periods. Whereas periods in isolated cells are generally either a few seconds or a few minutes, in intact islets of Langerhans they are intermediate (10-60 s). We develop a mathematical model for β-cell electrical activity capable of generating this wide range of bursting oscillations. Unlike previous models, bursting is driven by the interaction of two slow processes, one with a relatively small time constant (1-5 s) and the other with a much larger time constant (1-2 min). Bursting on the intermediate time scale is generated without need for a slow process having an intermediate time constant, hence phantom bursting. The model suggests that isolated cells exhibiting a fast pattern may nonetheless possess slower processes that can be brought out by injecting suitable exogenous currents. Guided by this, we devise an experimental protocol using the dynamic clamp technique that reliably elicits islet-like, medium period oscillations from isolated cells. Finally, we show that strong electrical coupling between a fast burster and a slow burster can produce synchronized medium bursting, suggesting that islets may be composed of cells that are intrinsically either fast or slow, with few or none that are intrinsically medium.

UR - http://www.scopus.com/inward/record.url?scp=0033634887&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033634887&partnerID=8YFLogxK

U2 - 10.1016/S0006-3495(00)76525-8

DO - 10.1016/S0006-3495(00)76525-8

M3 - Article

C2 - 11106596

AN - SCOPUS:0033634887

VL - 79

SP - 2880

EP - 2892

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 6

ER -