The platelet integrin alpha(IIb) beta(3) imaged by atomic force microscopy on model surfaces

Mohammad A. Hussain, Christopher A. Siedlecki

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The platelet membrane receptor αIIbβ3 binds to adsorbed protein ligands including fibrinogen, von Willebrand factor and fibronectin, and is critically important in mediating platelet adhesion to damaged subendothelium and to synthetic biomaterial surfaces. This receptor is a member of the integrin family, a highly prevalent class of heterodimeric molecules consisting of a single α and β subunit. In an ongoing effort to understand the mechanisms underlying platelet adhesion events, high-resolution atomic force microscopy (AFM) under dynamic conditions was used to obtain images of αIIbβ3 molecules as well as aggregates of the protein. Images of integrin molecules were obtained by tapping mode AFM under aqueous buffer conditions following adsorption on a series of ultrasmooth model surfaces. On a model hydrophobic surface, detergents stabilizing the protein in solution competed for surface adsorption sites. When this detergent was removed from the system, the protein was predominantly seen as aggregates with head groups pointing outward. A limited number of individual integrin molecules were observed, and were found to have dimensions consistent with those reported previously by electron microscopy studies. Integrin molecules showed weak adhesion to the two hydrophilic surfaces used in the study, although formation of a lipid bilayer around surface-adsorbed molecules improved the resolution. At longer time periods, the integrin molecules embedded in this lipid bilayer exhibited sufficient mobility to form molecular aggregates. The structural measurements described in this study not only reveal three-dimensional features of the molecule, they represent an important step towards dynamic adsorption experiments and visualizing the integrin interacting with surface-adsorbed proteins as in biomaterial-induced thrombogenesis.

Original languageEnglish (US)
Pages (from-to)565-573
Number of pages9
JournalMicron
Volume35
Issue number7
DOIs
StatePublished - Oct 1 2004

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Cell Biology

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