The predominant pathway of apoptosis in THP-1 macrophage-derived foam cells induced by 5-Aminolevulinic acid-mediated sonodynamic therapy is the mitochondria-caspase pathway despite the participation of endoplasmic reticulum stress

Huan Wang, Yang Yang, Haibo Chen, Juhua Dan, Jiali Cheng, Shuyuan Guo, Xin Sun, Wei Wang, Yiwei Ai, Shuchang Li, Zhixin Li, Li Peng, Zhen Tian, Liming Yang, Jichao Wu, Xin Zhong, Qi Zhou, Peng Wang, Zhiguo Zhang, Wenwu CaoYe Tian

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Background: In advanced atherosclerosis, chronic endoplasmic reticulum (ER) stress induces foam cells apoptosis and generates inflammatory reactions. Methods: THP-1 macrophage-derived foam cells (FC) were incubated with 1 mM 5-Aminolevulinic acid (ALA). After ALA mediated sonodynamic therapy (ALA-SDT), apoptosis of FC was assayed by Annexin V-PI staining. Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential were detected by staining with CellROX® Green Reagent and jc-1. Pretreatment of FC with N-Acetylcysteine (NAC), Z-VAD-FMK or 4-phenylbutyrate (4-PBA), mitochondria apoptotic pathway associated proteins and C/EBP-homologous (CHOP) expressions were assayed by wertern blotting. Results: Burst of apoptosis of FC was observed at 5-hour after ALA-SDT with 6-hour incubation of ALA and 0.4 W/cm 2 ultrasound. After ALA-SDT, intracellular ROS level increased and mitochondrial membrane potential collapsed. Translocations of cytochrome c from mitochondria into cytosol and Bax from cytosol into mitochondria, cleaved caspase 9, cleaved caspase 3, upregulation of CHOP, as well as downregulation of Bcl-2 after ALA-SDT were detected, which could be suppressed by NAC. Activation of mitochondria-caspase pathway could not be inhibited by 4-PBA. Cleaved caspase 9 and caspase 3 as well as apoptosis induced by ALA-SDT could be inhibited by Z-VAD-FMK. Conclusion: The mitochondria-caspase pathway is predominant in the apoptosis of FC induced by ALA-SDT though ER stress participates in.

Original languageEnglish (US)
Pages (from-to)1789-1801
Number of pages13
JournalCellular Physiology and Biochemistry
Volume33
Issue number6
DOIs
StatePublished - Jul 2014

All Science Journal Classification (ASJC) codes

  • Physiology

Fingerprint Dive into the research topics of 'The predominant pathway of apoptosis in THP-1 macrophage-derived foam cells induced by 5-Aminolevulinic acid-mediated sonodynamic therapy is the mitochondria-caspase pathway despite the participation of endoplasmic reticulum stress'. Together they form a unique fingerprint.

Cite this