The proinflammatory phenotype of PECAM-1-deficient mice results in atherogenic diet-induced steatohepatitis

Reema Goel, Brian Boylan, Lynn Gruman, Peter J. Newman, Paula E. North, Debra K. Newman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The severity of nonalcoholic steatohepatitis (NASH) is determined by environmental and genetic factors, the latter of which are incompletely characterized. Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a 130-kDa transmembrane glycoprotein expressed on blood and vascular cells. In the present study, we provide data for the novel finding that genetic deficiency of PECAM-1 potentiates the development and progression of NASH. We found that the rate of development and severity of diet-induced NASH are markedly enhanced in PECAM-1-deficient [knockout (KO)] mice relative to wild-type (WT) mice, as measured by histological and biochemical evaluation. Livers from KO mice exhibited typical histological features of NASH, including macrovesicular fat accumulation, hepatocyte injury with infiltration of inflammatory cells, fibrosis, and heightened oxidative stress. Alanine aminotransferase, a marker for liver injury, was also significantly higher in KO compared with WT mice. Consistent with a role for PECAM-1 as a suppressor of proinflammatory cytokines, plasma levels of inflammatory cytokines, including TNF-α and monocyte chemoattractant protein-1 (MCP-1), were also significantly higher in KO compared with WT mice. These findings are the first to show that the PECAM-1-deficient mouse develops progressive nonalcoholic fatty liver disease (NAFLD), supporting a role for PECAM-1 as a negative regulator of NAFLD progression. Future examination of recently identified PECAM-1 allelic isoforms in humans as potential risk factors for developing NASH may be warranted.

Original languageEnglish (US)
Pages (from-to)G1205-G1214
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume293
Issue number6
DOIs
StatePublished - Dec 1 2007

Fingerprint

CD31 Antigens
Atherogenic Diet
Fatty Liver
Phenotype
Knockout Mice
Cytokines
Chemokine CCL2
Liver
Wounds and Injuries
Non-alcoholic Fatty Liver Disease
Alanine Transaminase
Blood Vessels
Disease Progression
Hepatocytes
Blood Cells
Glycoproteins
Protein Isoforms
Oxidative Stress
Fibrosis
Fats

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

Goel, Reema ; Boylan, Brian ; Gruman, Lynn ; Newman, Peter J. ; North, Paula E. ; Newman, Debra K. / The proinflammatory phenotype of PECAM-1-deficient mice results in atherogenic diet-induced steatohepatitis. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2007 ; Vol. 293, No. 6. pp. G1205-G1214.
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The proinflammatory phenotype of PECAM-1-deficient mice results in atherogenic diet-induced steatohepatitis. / Goel, Reema; Boylan, Brian; Gruman, Lynn; Newman, Peter J.; North, Paula E.; Newman, Debra K.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 293, No. 6, 01.12.2007, p. G1205-G1214.

Research output: Contribution to journalArticle

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