The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies

Marisa S. Otegui, Rachel Herder, Jan Schulze, Rudolf Jung, L. Andrew Staehelin

Research output: Contribution to journalArticle

145 Scopus citations


We have investigated the transport of storage proteins, their processing proteases, and the Vacuolar Sorting Receptor-1/Epidermal Growth Factor Receptor-Like Protein1 (VSR-1/ATELP1) receptor during the formation of protein storage vacuoles in Arabidopsis thaliana embryos by means of high-pressure freezing/freeze substitution, electron tomography, immunolabeling techniques, and subcellular fractionation. The storage proteins and their processing proteases are segregated from each other within the Golgi cisternae and packaged into separate vesicles. The storage protein-containing vesicles but not the processing enzyme-containing vesicles carry the VSR-1/ATELP1 receptor. Both types of secretory vesicles appear to fuse into a type of prevacuolar multivesicular body (MVB). We have also determined that the proteolytic processing of the 2S albumins starts in the MVBs. We hypothesize that the compartmentalized processing of storage proteins in the MVBs may allow for the sequential activation of processing proteases as the MVB lumen gradually acidifies.

Original languageEnglish (US)
Pages (from-to)2567-2581
Number of pages15
JournalPlant Cell
Issue number10
StatePublished - Oct 1 2006


All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

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