The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum

Jun Miao, Jinfang Li, Qi Fan, Xiaolian Li, Xinyi Li, Liwang Cui

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Translation regulation plays an important role during gametocytogenesis in the malaria parasite, a process that is obligatory for the transmission of the parasite through mosquito vectors. In this study we determined the function of PfPuf2, a member of the Puf family of translational repressors, in gametocytogenesis of Plasmodium falciparum. Tagging of the endogenous PfPuf2 protein with green fluorescent protein showed that PfPuf2 was expressed in both male and female gametocytes, and the protein was localized in the cytoplasm of the parasite. Targeted disruption of the PfPuf2 gene did not affect asexual growth of the parasite, but promoted the formation of gametocytes and differentiation of male gametocytes. Complementation studies were performed to confirm that the resultant phenotypic changes were due to disruption of the PfPuf2 gene. Episomal expression of PfPuf2 under its cognate promoter almost restored the gametocytogenesis rate in a PfPuf2 disruptant to the level of the wild-type parasite. It also partially restored the effect of PfPuf2 disruption on male-female sex ratio. In addition, episomal overexpression of PfPuf2 under its cognate promoter but with a higher concentration of the selection drug or under the constitutive hsp86 promoter in both the PfPuf2-disruptant and wild-type 3D7 lines, further dramatically reduced gametocytogenesis rates and sex ratios. These findings suggest that in this early branch of eukaryotes the function of PfPuf2 is consistent with the ancestral function of suppressing differentiation proposed for Puf-family proteins.

Original languageEnglish (US)
Pages (from-to)1039-1049
Number of pages11
JournalJournal of Cell Science
Volume123
Issue number7
DOIs
StatePublished - Apr 1 2010

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Sex Differentiation
Sexual Development
RNA-Binding Proteins
Falciparum Malaria
Parasites
Sex Ratio
Proteins
Plasmodium falciparum
Green Fluorescent Proteins
Eukaryota
Genes
Malaria
Cytoplasm
Growth
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Miao, Jun ; Li, Jinfang ; Fan, Qi ; Li, Xiaolian ; Li, Xinyi ; Cui, Liwang. / The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum. In: Journal of Cell Science. 2010 ; Vol. 123, No. 7. pp. 1039-1049.
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The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum. / Miao, Jun; Li, Jinfang; Fan, Qi; Li, Xiaolian; Li, Xinyi; Cui, Liwang.

In: Journal of Cell Science, Vol. 123, No. 7, 01.04.2010, p. 1039-1049.

Research output: Contribution to journalArticle

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