Abstract

We have identified a cohort of zebrafish expressed sequence tags encoding eight Na,K-ATPase α subunits and five β subunits. Sequence comparisons and phylogenetic analysis indicate that five of the zebrafish α subunit genes comprise an α1-like gene subfamily and two are orthologs of the mammalian α3 subunit gene. The remaining α subunit clone is most similar to the mammalian α2 subunit. Among the five β subunit genes, two are orthologs of the mammalian β1 isoform, one represents a β2 ortholog, and two are orthologous to the mammalian β3 subunit. Using zebrafish radiation hybrid and meiotic mapping panels, we determined linkage assignments for each α and β subunit gene. Na,K-ATPase genes are dispersed in the zebrafish genome with the exception of four of the α1-like genes, which are tightly clustered on linkage group 1. Comparative mapping studies indicate that most of the zebrafish Na,K-ATPase genes localize to regions of conserved synteny between zebrafish and humans. The expression patterns of Na,K-ATPase α and β subunit genes in zebrafish are quite distinctive. No two α or β subunit genes exhibit the same expression profile. Together, our data imply a very high degree of Na, K-ATPase isoenzyme heterogeneity in zebrafish, with the potential for 40 structurally distinct α/β subunit combinations. Differences in expression patterns of α and β subunits suggest that many of the isoenzymes are also likely to exhibit differences in functional properties within specific cell and tissue types. Our studies form a framework for analyzing structure function relationships for sodium pump isoforms using reverse genetic approaches.

Original languageEnglish (US)
Pages (from-to)1211-1220
Number of pages10
JournalGenome research
Volume11
Issue number7
DOIs
StatePublished - Aug 6 2001

Fingerprint

Zebrafish
Genes
Isoenzymes
Protein Isoforms
sodium-translocating ATPase
Radiation Hybrid Mapping
Synteny
Reverse Genetics
Sodium-Potassium-Exchanging ATPase
Expressed Sequence Tags
Clone Cells
Genome

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Rajarao, S. Johannes R. ; Canfield, Victor A. ; Mohideen, Manzoor Ali P.K. ; Yan, Yi Lin ; Postlethwait, John H. ; Cheng, Keith C. ; Levenson, Robert. / The repertoire of Na, K-ATPase α and β subunit genes expressed in the Zebrafish, Danio rerio. In: Genome research. 2001 ; Vol. 11, No. 7. pp. 1211-1220.
@article{dd362ca250034cdd924db89ca2c0310c,
title = "The repertoire of Na, K-ATPase α and β subunit genes expressed in the Zebrafish, Danio rerio",
abstract = "We have identified a cohort of zebrafish expressed sequence tags encoding eight Na,K-ATPase α subunits and five β subunits. Sequence comparisons and phylogenetic analysis indicate that five of the zebrafish α subunit genes comprise an α1-like gene subfamily and two are orthologs of the mammalian α3 subunit gene. The remaining α subunit clone is most similar to the mammalian α2 subunit. Among the five β subunit genes, two are orthologs of the mammalian β1 isoform, one represents a β2 ortholog, and two are orthologous to the mammalian β3 subunit. Using zebrafish radiation hybrid and meiotic mapping panels, we determined linkage assignments for each α and β subunit gene. Na,K-ATPase genes are dispersed in the zebrafish genome with the exception of four of the α1-like genes, which are tightly clustered on linkage group 1. Comparative mapping studies indicate that most of the zebrafish Na,K-ATPase genes localize to regions of conserved synteny between zebrafish and humans. The expression patterns of Na,K-ATPase α and β subunit genes in zebrafish are quite distinctive. No two α or β subunit genes exhibit the same expression profile. Together, our data imply a very high degree of Na, K-ATPase isoenzyme heterogeneity in zebrafish, with the potential for 40 structurally distinct α/β subunit combinations. Differences in expression patterns of α and β subunits suggest that many of the isoenzymes are also likely to exhibit differences in functional properties within specific cell and tissue types. Our studies form a framework for analyzing structure function relationships for sodium pump isoforms using reverse genetic approaches.",
author = "Rajarao, {S. Johannes R.} and Canfield, {Victor A.} and Mohideen, {Manzoor Ali P.K.} and Yan, {Yi Lin} and Postlethwait, {John H.} and Cheng, {Keith C.} and Robert Levenson",
year = "2001",
month = "8",
day = "6",
doi = "10.1101/gr.186001",
language = "English (US)",
volume = "11",
pages = "1211--1220",
journal = "Genome Research",
issn = "1088-9051",
publisher = "Cold Spring Harbor Laboratory Press",
number = "7",

}

The repertoire of Na, K-ATPase α and β subunit genes expressed in the Zebrafish, Danio rerio. / Rajarao, S. Johannes R.; Canfield, Victor A.; Mohideen, Manzoor Ali P.K.; Yan, Yi Lin; Postlethwait, John H.; Cheng, Keith C.; Levenson, Robert.

In: Genome research, Vol. 11, No. 7, 06.08.2001, p. 1211-1220.

Research output: Contribution to journalLetter

TY - JOUR

T1 - The repertoire of Na, K-ATPase α and β subunit genes expressed in the Zebrafish, Danio rerio

AU - Rajarao, S. Johannes R.

AU - Canfield, Victor A.

AU - Mohideen, Manzoor Ali P.K.

AU - Yan, Yi Lin

AU - Postlethwait, John H.

AU - Cheng, Keith C.

AU - Levenson, Robert

PY - 2001/8/6

Y1 - 2001/8/6

N2 - We have identified a cohort of zebrafish expressed sequence tags encoding eight Na,K-ATPase α subunits and five β subunits. Sequence comparisons and phylogenetic analysis indicate that five of the zebrafish α subunit genes comprise an α1-like gene subfamily and two are orthologs of the mammalian α3 subunit gene. The remaining α subunit clone is most similar to the mammalian α2 subunit. Among the five β subunit genes, two are orthologs of the mammalian β1 isoform, one represents a β2 ortholog, and two are orthologous to the mammalian β3 subunit. Using zebrafish radiation hybrid and meiotic mapping panels, we determined linkage assignments for each α and β subunit gene. Na,K-ATPase genes are dispersed in the zebrafish genome with the exception of four of the α1-like genes, which are tightly clustered on linkage group 1. Comparative mapping studies indicate that most of the zebrafish Na,K-ATPase genes localize to regions of conserved synteny between zebrafish and humans. The expression patterns of Na,K-ATPase α and β subunit genes in zebrafish are quite distinctive. No two α or β subunit genes exhibit the same expression profile. Together, our data imply a very high degree of Na, K-ATPase isoenzyme heterogeneity in zebrafish, with the potential for 40 structurally distinct α/β subunit combinations. Differences in expression patterns of α and β subunits suggest that many of the isoenzymes are also likely to exhibit differences in functional properties within specific cell and tissue types. Our studies form a framework for analyzing structure function relationships for sodium pump isoforms using reverse genetic approaches.

AB - We have identified a cohort of zebrafish expressed sequence tags encoding eight Na,K-ATPase α subunits and five β subunits. Sequence comparisons and phylogenetic analysis indicate that five of the zebrafish α subunit genes comprise an α1-like gene subfamily and two are orthologs of the mammalian α3 subunit gene. The remaining α subunit clone is most similar to the mammalian α2 subunit. Among the five β subunit genes, two are orthologs of the mammalian β1 isoform, one represents a β2 ortholog, and two are orthologous to the mammalian β3 subunit. Using zebrafish radiation hybrid and meiotic mapping panels, we determined linkage assignments for each α and β subunit gene. Na,K-ATPase genes are dispersed in the zebrafish genome with the exception of four of the α1-like genes, which are tightly clustered on linkage group 1. Comparative mapping studies indicate that most of the zebrafish Na,K-ATPase genes localize to regions of conserved synteny between zebrafish and humans. The expression patterns of Na,K-ATPase α and β subunit genes in zebrafish are quite distinctive. No two α or β subunit genes exhibit the same expression profile. Together, our data imply a very high degree of Na, K-ATPase isoenzyme heterogeneity in zebrafish, with the potential for 40 structurally distinct α/β subunit combinations. Differences in expression patterns of α and β subunits suggest that many of the isoenzymes are also likely to exhibit differences in functional properties within specific cell and tissue types. Our studies form a framework for analyzing structure function relationships for sodium pump isoforms using reverse genetic approaches.

UR - http://www.scopus.com/inward/record.url?scp=0034912319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034912319&partnerID=8YFLogxK

U2 - 10.1101/gr.186001

DO - 10.1101/gr.186001

M3 - Letter

C2 - 11435403

AN - SCOPUS:0034912319

VL - 11

SP - 1211

EP - 1220

JO - Genome Research

JF - Genome Research

SN - 1088-9051

IS - 7

ER -