The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance

Haijun Zhao, Xueyan Wang, Yulin Jia, Bastian Minkenberg, Matthew Wheatley, Jiangbo Fan, Melissa H. Jia, Adam Famoso, Jeremy D. Edwards, Yeshi Wamishe, Barbara Valent, Guo Liang Wang, Yinong Yang

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34 Scopus citations

Abstract

Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm. A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.

Original languageEnglish (US)
Article number4369
JournalNature communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Zhao, H., Wang, X., Jia, Y., Minkenberg, B., Wheatley, M., Fan, J., Jia, M. H., Famoso, A., Edwards, J. D., Wamishe, Y., Valent, B., Wang, G. L., & Yang, Y. (2018). The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance. Nature communications, 9(1), [4369]. https://doi.org/10.1038/s41467-018-04369-4