The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance

Haijun Zhao, Xueyan Wang, Yulin Jia, Bastian Minkenberg, Matthew Wheatley, Jiangbo Fan, Melissa H. Jia, Adam Famoso, Jeremy D. Edwards, Yeshi Wamishe, Barbara Valent, Guo Liang Wang, Yinong Yang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm. A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.

Original languageEnglish (US)
Article number4369
JournalNature communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

Blast resistance
Disease Resistance
rice
blasts
genes
vpr Genes
Genes
proteins
Clustered Regularly Interspaced Short Palindromic Repeats
Armadillos
Fast Neutrons
Plant Genes
coding
Proteins
Gene encoding
Cloning
magnetic permeability
Leucine
Base Pairing
deletion

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Zhao, Haijun ; Wang, Xueyan ; Jia, Yulin ; Minkenberg, Bastian ; Wheatley, Matthew ; Fan, Jiangbo ; Jia, Melissa H. ; Famoso, Adam ; Edwards, Jeremy D. ; Wamishe, Yeshi ; Valent, Barbara ; Wang, Guo Liang ; Yang, Yinong. / The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance. In: Nature communications. 2018 ; Vol. 9, No. 1.
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abstract = "Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm. A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.",
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Zhao, H, Wang, X, Jia, Y, Minkenberg, B, Wheatley, M, Fan, J, Jia, MH, Famoso, A, Edwards, JD, Wamishe, Y, Valent, B, Wang, GL & Yang, Y 2018, 'The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance' Nature communications, vol. 9, no. 1, 4369. https://doi.org/10.1038/s41467-018-04369-4

The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance. / Zhao, Haijun; Wang, Xueyan; Jia, Yulin; Minkenberg, Bastian; Wheatley, Matthew; Fan, Jiangbo; Jia, Melissa H.; Famoso, Adam; Edwards, Jeremy D.; Wamishe, Yeshi; Valent, Barbara; Wang, Guo Liang; Yang, Yinong.

In: Nature communications, Vol. 9, No. 1, 4369, 01.12.2018.

Research output: Contribution to journalArticle

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T1 - The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance

AU - Zhao, Haijun

AU - Wang, Xueyan

AU - Jia, Yulin

AU - Minkenberg, Bastian

AU - Wheatley, Matthew

AU - Fan, Jiangbo

AU - Jia, Melissa H.

AU - Famoso, Adam

AU - Edwards, Jeremy D.

AU - Wamishe, Yeshi

AU - Valent, Barbara

AU - Wang, Guo Liang

AU - Yang, Yinong

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Y1 - 2018/12/1

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AB - Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm. A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.

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