@article{439aba43724245aba58fd44942c092a4,
title = "The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance",
abstract = "Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm. A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.",
author = "Haijun Zhao and Xueyan Wang and Yulin Jia and Bastian Minkenberg and Matthew Wheatley and Jiangbo Fan and Jia, {Melissa H.} and Adam Famoso and Edwards, {Jeremy D.} and Yeshi Wamishe and Barbara Valent and Wang, {Guo Liang} and Yinong Yang",
note = "Funding Information: We thank Michael Lin and Tracy Bianco and other members of USDA ARS Dale Bumpers National Rice Research Center and Drs. Miles Lange, and Adam Fuller and their supporting staff members of Harry K. Dupree Stuttgart National Aquaculture Research Center, Amber Delhagen of Pennsylvania State University, Melinda Dalby of Kansas State University, Drs. Lirong Zeng and Bangjun Zhou of University of Nebraska, and Xinglong Chen of China Agricultural University for their excellent technical assistance; and Fanny Liu and Brian Scheffler at USDA ARS Stoneville for all DNA sequencing. For critical reading of this manuscript, we thank Roger Thilmony, Dennis Halterman, Xianming Chen, and Anna McClung of USDA ARS. This project in part is supported by Agriculture and Food Research Initiative Competitive Grant number 2013-68004-20378 (Blast Integrated Project, “BIP”) from the USDA National Institute of Food and Agriculture, USDA-Agricultural Research Service NP301 in-house project, Louisiana Rice Research Board, and The OARDC Research Enhancement Competitive Grants Program (SEEDS) at the Ohio State University. This is contribution number 18-192-J from the Kansas Agricultural Experiment Station. USDA is an equal opportunity employer and provider. Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41467-018-04369-4",
language = "English (US)",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}