The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development

Na Li, Da Sheng Zhang, Hai Sheng Liu, Chang Song Yin, Xiao Xing Li, Wan Qi Liang, Zheng Yuan, Ben Xu, Huang Wei Chu, Jia Wang, Tie Qiao Wen, Hai Huang, Da Luo, Hong Ma, Da Bing Zhang

Research output: Contribution to journalArticle

345 Citations (Scopus)

Abstract

In flowering plants, tapetum degeneration is proposed to be triggered by a programmed cell death (PCD) process during late stages of pollen development; the PCD is thought to provide cellular contents supporting pollen wall formation and to allow the subsequent pollen release. However, the molecular basis regulating tapetum PCD in plants remains poorly understood. We report the isolation and characterization of a rice (Oryza sativa) male sterile mutant tapetum degeneration retardation (tdr), which exhibits degeneration retardation of the tapetum and middle layer as well as collapse of microspores. The TDR gene is preferentially expressed in the tapetum and encodes a putative basic helix-loop-helix protein, which is likely localized to the nucleus. More importantly, two genes, Os CP1 and Os c6, encoding a Cys protease and a protease inhibitor, respectively, were shown to be the likely direct targets of TDR through chromatin immunoprecipitation analyses and the electrophoretic mobility shift assay. These results indicate that TDR is a key component of the molecular network regulating rice tapetum development and degeneration.

Original languageEnglish (US)
Pages (from-to)2999-3014
Number of pages16
JournalPlant Cell
Volume18
Issue number11
DOIs
StatePublished - Nov 1 2006

Fingerprint

Pollen
anthers
Cell Death
apoptosis
pollen
rice
degradation
Genes
genes
Chromatin Immunoprecipitation
Electrophoretic Mobility Shift Assay
microspores
proteinase inhibitors
Protease Inhibitors
chromatin
Angiospermae
Oryza sativa
Peptide Hydrolases
proteinases
mutants

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Li, N., Zhang, D. S., Liu, H. S., Yin, C. S., Li, X. X., Liang, W. Q., ... Zhang, D. B. (2006). The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development. Plant Cell, 18(11), 2999-3014. https://doi.org/10.1105/tpc.106.044107
Li, Na ; Zhang, Da Sheng ; Liu, Hai Sheng ; Yin, Chang Song ; Li, Xiao Xing ; Liang, Wan Qi ; Yuan, Zheng ; Xu, Ben ; Chu, Huang Wei ; Wang, Jia ; Wen, Tie Qiao ; Huang, Hai ; Luo, Da ; Ma, Hong ; Zhang, Da Bing. / The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development. In: Plant Cell. 2006 ; Vol. 18, No. 11. pp. 2999-3014.
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abstract = "In flowering plants, tapetum degeneration is proposed to be triggered by a programmed cell death (PCD) process during late stages of pollen development; the PCD is thought to provide cellular contents supporting pollen wall formation and to allow the subsequent pollen release. However, the molecular basis regulating tapetum PCD in plants remains poorly understood. We report the isolation and characterization of a rice (Oryza sativa) male sterile mutant tapetum degeneration retardation (tdr), which exhibits degeneration retardation of the tapetum and middle layer as well as collapse of microspores. The TDR gene is preferentially expressed in the tapetum and encodes a putative basic helix-loop-helix protein, which is likely localized to the nucleus. More importantly, two genes, Os CP1 and Os c6, encoding a Cys protease and a protease inhibitor, respectively, were shown to be the likely direct targets of TDR through chromatin immunoprecipitation analyses and the electrophoretic mobility shift assay. These results indicate that TDR is a key component of the molecular network regulating rice tapetum development and degeneration.",
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Li, N, Zhang, DS, Liu, HS, Yin, CS, Li, XX, Liang, WQ, Yuan, Z, Xu, B, Chu, HW, Wang, J, Wen, TQ, Huang, H, Luo, D, Ma, H & Zhang, DB 2006, 'The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development', Plant Cell, vol. 18, no. 11, pp. 2999-3014. https://doi.org/10.1105/tpc.106.044107

The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development. / Li, Na; Zhang, Da Sheng; Liu, Hai Sheng; Yin, Chang Song; Li, Xiao Xing; Liang, Wan Qi; Yuan, Zheng; Xu, Ben; Chu, Huang Wei; Wang, Jia; Wen, Tie Qiao; Huang, Hai; Luo, Da; Ma, Hong; Zhang, Da Bing.

In: Plant Cell, Vol. 18, No. 11, 01.11.2006, p. 2999-3014.

Research output: Contribution to journalArticle

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T1 - The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development

AU - Li, Na

AU - Zhang, Da Sheng

AU - Liu, Hai Sheng

AU - Yin, Chang Song

AU - Li, Xiao Xing

AU - Liang, Wan Qi

AU - Yuan, Zheng

AU - Xu, Ben

AU - Chu, Huang Wei

AU - Wang, Jia

AU - Wen, Tie Qiao

AU - Huang, Hai

AU - Luo, Da

AU - Ma, Hong

AU - Zhang, Da Bing

PY - 2006/11/1

Y1 - 2006/11/1

N2 - In flowering plants, tapetum degeneration is proposed to be triggered by a programmed cell death (PCD) process during late stages of pollen development; the PCD is thought to provide cellular contents supporting pollen wall formation and to allow the subsequent pollen release. However, the molecular basis regulating tapetum PCD in plants remains poorly understood. We report the isolation and characterization of a rice (Oryza sativa) male sterile mutant tapetum degeneration retardation (tdr), which exhibits degeneration retardation of the tapetum and middle layer as well as collapse of microspores. The TDR gene is preferentially expressed in the tapetum and encodes a putative basic helix-loop-helix protein, which is likely localized to the nucleus. More importantly, two genes, Os CP1 and Os c6, encoding a Cys protease and a protease inhibitor, respectively, were shown to be the likely direct targets of TDR through chromatin immunoprecipitation analyses and the electrophoretic mobility shift assay. These results indicate that TDR is a key component of the molecular network regulating rice tapetum development and degeneration.

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