The RNA structurome: Transcriptome-wide structure probing with next-generation sequencing

Chun Kit Kwok, Yin Tang, Sarah M. Assmann, Philip C. Bevilacqua

Research output: Contribution to journalReview articlepeer-review

98 Scopus citations


RNA folds into intricate structures that enable its pivotal roles in biology, ranging from regulation of gene expression to ligand sensing and enzymatic functions. Therefore, elucidating RNA structure can provide profound insights into living systems. A recent marriage between in vivo RNA structure probing and next-generation sequencing (NGS) has revolutionized the RNA field by enabling transcriptome-wide structure determination in vivo, which has been applied to date to human cells, yeast cells, and Arabidopsis seedlings. Analysis of resultant in vivo 'RNA structuromes' provides new and important information regarding myriad cellular processes, including control of translation, alternative splicing, alternative polyadenylation, energy-dependent unfolding of mRNA, and effects of proteins on RNA structure. An emerging view suggests potential links between RNA structure and stress and disease physiology across the tree of life. As we discuss here, these exciting findings open new frontiers into RNA biology, genome biology, and beyond.

Original languageEnglish (US)
Pages (from-to)221-232
Number of pages12
JournalTrends in Biochemical Sciences
Issue number4
StatePublished - Apr 1 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


Dive into the research topics of 'The RNA structurome: Transcriptome-wide structure probing with next-generation sequencing'. Together they form a unique fingerprint.

Cite this