The role of canonical transient receptor potential 7 in B-cell receptor-activated channels

Jean Philippe Lievremont, Takuro Numaga, Guillermo Vazquez, Loïc Lemonnier, Yuji Hara, Emiko Mori, Mohamed Trebak, Stephen E. Moss, Gary S. Bird, Yasuo Mori, James W. Putney

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Phospholipase C signaling stimulates Ca2+ entry across the plasma membrane through multiple mechanisms. Ca2+ store depletion stimulates store-operated Ca2+-selective channels, or alternatively, other phospholipase C-dependent events activate Ca2+-permeable non-selective cation channels. Transient receptor potential 7 (TRPC7) is a non-selective cation channel that can be activated by both mechanisms when ectopically expressed, but the regulation of native TRPC7 channels is not known. We knocked out TRPC7 in DT40 B-cells, which expresses both forms of Ca 2+ entry. No difference in the store-operated current I crac was detected between TRPC7-/- and wild-type cells. Wild-type cells demonstrated non-store-operated cation entry and currents in response to activation of the B-cell receptor or protease-activated receptor 2, intracellular dialysis with GTPγS, or application of the synthetic diacylglycerol oleyl-acetyl-glycerol. These responses were absent in TRPC7 -/- cells but could be restored by transfection with human TRPC7. In conclusion, in B-lymphocytes, TRPC7 appeared to participate in the formation of ion channels that could be activated by phospholipase C-linked receptors. This represents the first demonstration of a physiological function for endogenous TRPC7 channels.

Original languageEnglish (US)
Pages (from-to)35346-35351
Number of pages6
JournalJournal of Biological Chemistry
Volume280
Issue number42
DOIs
StatePublished - Oct 21 2005

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Type C Phospholipases
Cations
B-Lymphocytes
Cells
PAR-2 Receptor
Dialysis
Lymphocytes
Diglycerides
Cell membranes
Ion Channels
Transfection
Demonstrations
Chemical activation
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lievremont, J. P., Numaga, T., Vazquez, G., Lemonnier, L., Hara, Y., Mori, E., ... Putney, J. W. (2005). The role of canonical transient receptor potential 7 in B-cell receptor-activated channels. Journal of Biological Chemistry, 280(42), 35346-35351. https://doi.org/10.1074/jbc.M507606200
Lievremont, Jean Philippe ; Numaga, Takuro ; Vazquez, Guillermo ; Lemonnier, Loïc ; Hara, Yuji ; Mori, Emiko ; Trebak, Mohamed ; Moss, Stephen E. ; Bird, Gary S. ; Mori, Yasuo ; Putney, James W. / The role of canonical transient receptor potential 7 in B-cell receptor-activated channels. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 42. pp. 35346-35351.
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Lievremont, JP, Numaga, T, Vazquez, G, Lemonnier, L, Hara, Y, Mori, E, Trebak, M, Moss, SE, Bird, GS, Mori, Y & Putney, JW 2005, 'The role of canonical transient receptor potential 7 in B-cell receptor-activated channels', Journal of Biological Chemistry, vol. 280, no. 42, pp. 35346-35351. https://doi.org/10.1074/jbc.M507606200

The role of canonical transient receptor potential 7 in B-cell receptor-activated channels. / Lievremont, Jean Philippe; Numaga, Takuro; Vazquez, Guillermo; Lemonnier, Loïc; Hara, Yuji; Mori, Emiko; Trebak, Mohamed; Moss, Stephen E.; Bird, Gary S.; Mori, Yasuo; Putney, James W.

In: Journal of Biological Chemistry, Vol. 280, No. 42, 21.10.2005, p. 35346-35351.

Research output: Contribution to journalArticle

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T1 - The role of canonical transient receptor potential 7 in B-cell receptor-activated channels

AU - Lievremont, Jean Philippe

AU - Numaga, Takuro

AU - Vazquez, Guillermo

AU - Lemonnier, Loïc

AU - Hara, Yuji

AU - Mori, Emiko

AU - Trebak, Mohamed

AU - Moss, Stephen E.

AU - Bird, Gary S.

AU - Mori, Yasuo

AU - Putney, James W.

PY - 2005/10/21

Y1 - 2005/10/21

N2 - Phospholipase C signaling stimulates Ca2+ entry across the plasma membrane through multiple mechanisms. Ca2+ store depletion stimulates store-operated Ca2+-selective channels, or alternatively, other phospholipase C-dependent events activate Ca2+-permeable non-selective cation channels. Transient receptor potential 7 (TRPC7) is a non-selective cation channel that can be activated by both mechanisms when ectopically expressed, but the regulation of native TRPC7 channels is not known. We knocked out TRPC7 in DT40 B-cells, which expresses both forms of Ca 2+ entry. No difference in the store-operated current I crac was detected between TRPC7-/- and wild-type cells. Wild-type cells demonstrated non-store-operated cation entry and currents in response to activation of the B-cell receptor or protease-activated receptor 2, intracellular dialysis with GTPγS, or application of the synthetic diacylglycerol oleyl-acetyl-glycerol. These responses were absent in TRPC7 -/- cells but could be restored by transfection with human TRPC7. In conclusion, in B-lymphocytes, TRPC7 appeared to participate in the formation of ion channels that could be activated by phospholipase C-linked receptors. This represents the first demonstration of a physiological function for endogenous TRPC7 channels.

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Lievremont JP, Numaga T, Vazquez G, Lemonnier L, Hara Y, Mori E et al. The role of canonical transient receptor potential 7 in B-cell receptor-activated channels. Journal of Biological Chemistry. 2005 Oct 21;280(42):35346-35351. https://doi.org/10.1074/jbc.M507606200