The role of liquid mixing and gas-phase dispersion in a submerged, sparged root reactor

Lia D. Tescione, Divakar Ramakrishnan, Wayne R. Curtis

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

An Agrobacterium-transformed root culture of Solanum tuberosum was grown in a 15-l bubble column. The specific respiration rate decreased by a factor of ten as the tissue grew over a 25-day culture period. On days 5, 8, 13, and 21, respiration was shown to be independent of aeration rate over a range of 0.05-0.4 vvm (volume of air per volume of liquid min-1). Gas dispersion measured from argon tracer residence time distributions increased four-fold due to increased stagnation and channeling of gas through the bed of growing roots; however, introduction of an antifoam surfactant on day 20 greatly reduced dispersion with no accompanying change in respiration. Taken together, the gas dispersion and respiration studies suggest that the gas-liquid interface is not the dominant resistance to oxygen mass transfer. Liquid mixing time measured with a dye tracer increased from 1.45 ± 0.45 min with no root tissue to 40.2 ± 1.6 min with 180 g FW l-1 of roots in the column. In addition, the oxygen uptake rate of growing growing tips (5.2 ± 0.2 mm) of individual root segments of S. tuberosum measured in a stirred microcell (600 μl) increased with the oxygen tension of the medium. Based on these results, the role of liquid mixing, gas-phase dispersion, and diffusion in the tissue in the scaleup of root culture is discussed.

Original languageEnglish (US)
Pages (from-to)207-213
Number of pages7
JournalEnzyme and Microbial Technology
Volume20
Issue number3
DOIs
StatePublished - Feb 15 1997

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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