TY - JOUR
T1 - The role of liquid mixing and gas-phase dispersion in a submerged, sparged root reactor
AU - Tescione, Lia D.
AU - Ramakrishnan, Divakar
AU - Curtis, Wayne R.
N1 - Funding Information:
The authorsw ould like to thankE dgardC arvalho,D erek Luyk, Don Lucas, and Kathy Petersf or their assistance. This work was supportedby the NationalS cienceF ounda-tion (Grantn o. BCS-9110288)a nd an NSF Young Inves-tigatorA ward (BCS-9358452)O. ne of the authors( LDT) was supportedb y the NIH BiotechnologyT raining Grant (Grantn o. GM0835842).
PY - 1997/2/15
Y1 - 1997/2/15
N2 - An Agrobacterium-transformed root culture of Solanum tuberosum was grown in a 15-l bubble column. The specific respiration rate decreased by a factor of ten as the tissue grew over a 25-day culture period. On days 5, 8, 13, and 21, respiration was shown to be independent of aeration rate over a range of 0.05-0.4 vvm (volume of air per volume of liquid min-1). Gas dispersion measured from argon tracer residence time distributions increased four-fold due to increased stagnation and channeling of gas through the bed of growing roots; however, introduction of an antifoam surfactant on day 20 greatly reduced dispersion with no accompanying change in respiration. Taken together, the gas dispersion and respiration studies suggest that the gas-liquid interface is not the dominant resistance to oxygen mass transfer. Liquid mixing time measured with a dye tracer increased from 1.45 ± 0.45 min with no root tissue to 40.2 ± 1.6 min with 180 g FW l-1 of roots in the column. In addition, the oxygen uptake rate of growing growing tips (5.2 ± 0.2 mm) of individual root segments of S. tuberosum measured in a stirred microcell (600 μl) increased with the oxygen tension of the medium. Based on these results, the role of liquid mixing, gas-phase dispersion, and diffusion in the tissue in the scaleup of root culture is discussed.
AB - An Agrobacterium-transformed root culture of Solanum tuberosum was grown in a 15-l bubble column. The specific respiration rate decreased by a factor of ten as the tissue grew over a 25-day culture period. On days 5, 8, 13, and 21, respiration was shown to be independent of aeration rate over a range of 0.05-0.4 vvm (volume of air per volume of liquid min-1). Gas dispersion measured from argon tracer residence time distributions increased four-fold due to increased stagnation and channeling of gas through the bed of growing roots; however, introduction of an antifoam surfactant on day 20 greatly reduced dispersion with no accompanying change in respiration. Taken together, the gas dispersion and respiration studies suggest that the gas-liquid interface is not the dominant resistance to oxygen mass transfer. Liquid mixing time measured with a dye tracer increased from 1.45 ± 0.45 min with no root tissue to 40.2 ± 1.6 min with 180 g FW l-1 of roots in the column. In addition, the oxygen uptake rate of growing growing tips (5.2 ± 0.2 mm) of individual root segments of S. tuberosum measured in a stirred microcell (600 μl) increased with the oxygen tension of the medium. Based on these results, the role of liquid mixing, gas-phase dispersion, and diffusion in the tissue in the scaleup of root culture is discussed.
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U2 - 10.1016/S0141-0229(96)00112-3
DO - 10.1016/S0141-0229(96)00112-3
M3 - Article
C2 - 9090955
AN - SCOPUS:0031568798
SN - 0141-0229
VL - 20
SP - 207
EP - 213
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 3
ER -