The role of oriT in tra-dependent enhanced recombination between mini-F-lac-oriT and λplac5

Jeffrey R. Carter, Dipty R. Patel, Ronald D. Porter

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Recombination between F42lac and λplac5 is typically 20- to 50-fold more efficient than recombination between chromosomal lac and λplac5. This enhancement of recombination is recBCD-dependent and requires the expression of genes from the tra regulon of the F factor. Also required is oriT, the origin of F factor conjugational transfer, which must be located in-cis to the cellular copy of lac. In this study we show that enhanced recombination is not supported by an oriT point mutant that reduces oriT function in conjugation. We also present evidence that the activation of oriT for recombination enhancement involves the same strand-specific nick that is required for conjugal DNA transfer. Although it is thought that the role of oriT in recombination enhancement is related to the facilitated entry of RecBCD enzyme into the DNA duplex, we were unable to detect any double-strand breakage at oriT.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalGenetical research
Volume59
Issue number3
DOIs
StatePublished - Jun 1992

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'The role of oriT in tra-dependent enhanced recombination between mini-F-lac-oriT and λplac5'. Together they form a unique fingerprint.

Cite this