The role of the NKG2D immunoreceptor in immune cell activation and natural killing

Amanda M. Jamieson, Andreas Diefenbach, Christopher W. McMahon, Na Xiong, James R. Carlyle, David H. Raulet

Research output: Contribution to journalArticle

442 Citations (Scopus)

Abstract

Little is known concerning the stimulatory receptors responsible for tumor cell lysis by NK cells. We generated a monoclonal antibody specific for murine NKG2D in order to investigate its function. Blocking of NKG2D inhibited natural cytotoxicity of all tumor cells tested that express ligands for the receptor. Staining analysis showed that NKG2D is also expressed by activated CD8+ T cells and macrophages, and subsets of TCRγδ+ and NK1.1+ T cells. Contradicting reports that NKG2D is solely a costimulatory receptor, we observed that cross-linking of NKG2D directly stimulates NK cells and activated macrophages. In contrast, NKG2D costimulates activated CD8+ T cells. Thus, NKG2D engagement directly stimulates NK cells and macrophages, costimulates CD8+ T cells, and plays a substantial role in natural killing.

Original languageEnglish (US)
Pages (from-to)19-29
Number of pages11
JournalImmunity
Volume17
Issue number1
DOIs
StatePublished - Jan 1 2002

Fingerprint

Natural Killer Cells
Macrophages
T-Lymphocytes
T-Lymphocyte Subsets
Neoplasms
Monoclonal Antibodies
Staining and Labeling
Ligands

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Jamieson, A. M., Diefenbach, A., McMahon, C. W., Xiong, N., Carlyle, J. R., & Raulet, D. H. (2002). The role of the NKG2D immunoreceptor in immune cell activation and natural killing. Immunity, 17(1), 19-29. https://doi.org/10.1016/S1074-7613(02)00333-3
Jamieson, Amanda M. ; Diefenbach, Andreas ; McMahon, Christopher W. ; Xiong, Na ; Carlyle, James R. ; Raulet, David H. / The role of the NKG2D immunoreceptor in immune cell activation and natural killing. In: Immunity. 2002 ; Vol. 17, No. 1. pp. 19-29.
@article{cd1d0e693e074315ae8208638abedb36,
title = "The role of the NKG2D immunoreceptor in immune cell activation and natural killing",
abstract = "Little is known concerning the stimulatory receptors responsible for tumor cell lysis by NK cells. We generated a monoclonal antibody specific for murine NKG2D in order to investigate its function. Blocking of NKG2D inhibited natural cytotoxicity of all tumor cells tested that express ligands for the receptor. Staining analysis showed that NKG2D is also expressed by activated CD8+ T cells and macrophages, and subsets of TCRγδ+ and NK1.1+ T cells. Contradicting reports that NKG2D is solely a costimulatory receptor, we observed that cross-linking of NKG2D directly stimulates NK cells and activated macrophages. In contrast, NKG2D costimulates activated CD8+ T cells. Thus, NKG2D engagement directly stimulates NK cells and macrophages, costimulates CD8+ T cells, and plays a substantial role in natural killing.",
author = "Jamieson, {Amanda M.} and Andreas Diefenbach and McMahon, {Christopher W.} and Na Xiong and Carlyle, {James R.} and Raulet, {David H.}",
year = "2002",
month = "1",
day = "1",
doi = "10.1016/S1074-7613(02)00333-3",
language = "English (US)",
volume = "17",
pages = "19--29",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "1",

}

Jamieson, AM, Diefenbach, A, McMahon, CW, Xiong, N, Carlyle, JR & Raulet, DH 2002, 'The role of the NKG2D immunoreceptor in immune cell activation and natural killing', Immunity, vol. 17, no. 1, pp. 19-29. https://doi.org/10.1016/S1074-7613(02)00333-3

The role of the NKG2D immunoreceptor in immune cell activation and natural killing. / Jamieson, Amanda M.; Diefenbach, Andreas; McMahon, Christopher W.; Xiong, Na; Carlyle, James R.; Raulet, David H.

In: Immunity, Vol. 17, No. 1, 01.01.2002, p. 19-29.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The role of the NKG2D immunoreceptor in immune cell activation and natural killing

AU - Jamieson, Amanda M.

AU - Diefenbach, Andreas

AU - McMahon, Christopher W.

AU - Xiong, Na

AU - Carlyle, James R.

AU - Raulet, David H.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Little is known concerning the stimulatory receptors responsible for tumor cell lysis by NK cells. We generated a monoclonal antibody specific for murine NKG2D in order to investigate its function. Blocking of NKG2D inhibited natural cytotoxicity of all tumor cells tested that express ligands for the receptor. Staining analysis showed that NKG2D is also expressed by activated CD8+ T cells and macrophages, and subsets of TCRγδ+ and NK1.1+ T cells. Contradicting reports that NKG2D is solely a costimulatory receptor, we observed that cross-linking of NKG2D directly stimulates NK cells and activated macrophages. In contrast, NKG2D costimulates activated CD8+ T cells. Thus, NKG2D engagement directly stimulates NK cells and macrophages, costimulates CD8+ T cells, and plays a substantial role in natural killing.

AB - Little is known concerning the stimulatory receptors responsible for tumor cell lysis by NK cells. We generated a monoclonal antibody specific for murine NKG2D in order to investigate its function. Blocking of NKG2D inhibited natural cytotoxicity of all tumor cells tested that express ligands for the receptor. Staining analysis showed that NKG2D is also expressed by activated CD8+ T cells and macrophages, and subsets of TCRγδ+ and NK1.1+ T cells. Contradicting reports that NKG2D is solely a costimulatory receptor, we observed that cross-linking of NKG2D directly stimulates NK cells and activated macrophages. In contrast, NKG2D costimulates activated CD8+ T cells. Thus, NKG2D engagement directly stimulates NK cells and macrophages, costimulates CD8+ T cells, and plays a substantial role in natural killing.

UR - http://www.scopus.com/inward/record.url?scp=0036333884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036333884&partnerID=8YFLogxK

U2 - 10.1016/S1074-7613(02)00333-3

DO - 10.1016/S1074-7613(02)00333-3

M3 - Article

C2 - 12150888

AN - SCOPUS:0036333884

VL - 17

SP - 19

EP - 29

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 1

ER -

Jamieson AM, Diefenbach A, McMahon CW, Xiong N, Carlyle JR, Raulet DH. The role of the NKG2D immunoreceptor in immune cell activation and natural killing. Immunity. 2002 Jan 1;17(1):19-29. https://doi.org/10.1016/S1074-7613(02)00333-3