TY - JOUR
T1 - The roles of CRISPR-Cas systems in adaptive immunity and beyond
AU - Barrangou, Rodolphe
N1 - Funding Information:
RB is supported by start up funds from North Carolina State University . The author would like to thank his many colleagues and collaborators in the CRISPR field for their insights into these fantastic molecular systems.
Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying Cas proteins constitute the adaptive CRISPR-Cas immune system in bacteria and archaea. This DNA-encoded, RNA-mediated defense system provides sequence-specific recognition, targeting and degradation of exogenous nucleic acid. Though the primary established role of CRISPR-Cas systems is in bona fide adaptive antiviral defense in bacteria, a growing body of evidence indicates that it also plays critical functional roles beyond immunity, such as endogenous transcriptional control. Furthermore, benefits inherent to maintaining genome homeostasis also come at the cost of reduced uptake of beneficial DNA, and preventing strategic adaptation to the environment. This opens new avenues for the investigation of CRISPR-Cas systems and their functional characterization beyond adaptive immunity.
AB - Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying Cas proteins constitute the adaptive CRISPR-Cas immune system in bacteria and archaea. This DNA-encoded, RNA-mediated defense system provides sequence-specific recognition, targeting and degradation of exogenous nucleic acid. Though the primary established role of CRISPR-Cas systems is in bona fide adaptive antiviral defense in bacteria, a growing body of evidence indicates that it also plays critical functional roles beyond immunity, such as endogenous transcriptional control. Furthermore, benefits inherent to maintaining genome homeostasis also come at the cost of reduced uptake of beneficial DNA, and preventing strategic adaptation to the environment. This opens new avenues for the investigation of CRISPR-Cas systems and their functional characterization beyond adaptive immunity.
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U2 - 10.1016/j.coi.2014.12.008
DO - 10.1016/j.coi.2014.12.008
M3 - Review article
C2 - 25574773
AN - SCOPUS:84920871112
VL - 32
SP - 36
EP - 41
JO - Current Opinion in Immunology
JF - Current Opinion in Immunology
SN - 0952-7915
ER -