The Saccharomyces cerevisiae Recombination Enhancer Biases Recombination during Interchromosomal Mating-Type Switching but Not in Interchromosomal Homologous Recombination

Peter Houston, Peter J. Simon, James R. Broach

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Haploid Saccharomyces can change mating type through HO-endonuclease cleavage of an expressor locus, MAT, followed by gene conversion using one of two repository loci, HML or HMR, as donor. The mating type of a cell dictates which repository locus is used as donor, with a cells using HML and α cells using HMR. This preference is established in part by RE, a locus on the left arm of chromosome III that activates the surrounding region, including HML, for recombination in a cells, an activity suppressed by α2 protein in α cells. We have examined the ability of RE to stimulate different forms of interchromosomal recombination. We found that RE exerted an effect on interchromosomal mating-type switching and on intrachromosomal homologous recombination but not on interchromosomal homologous recombination. Also, even in the absence of RE, MATα still influenced donor preference in interchromosomal mating-type switching, supporting a role of α2 in donor preference independent of RE. These results suggest a model in which RE affects competition between productive and nonproductive recombination outcomes. In interchromosome gene conversion, RE enhances both productive and nonproductive pathways, whereas in intrachromosomal gene conversion and mating-type switching, RE enhances only the productive pathway.

Original languageEnglish (US)
Pages (from-to)1187-1197
Number of pages11
JournalGenetics
Volume166
Issue number3
DOIs
StatePublished - Mar 2004

All Science Journal Classification (ASJC) codes

  • Genetics

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