The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally

Varinia García-Molinero, José García-Martínez, Rohit Reja, Pedro Furió-Tarí, Oreto Antúnez, Vinesh Vinayachandran, Ana Conesa, B. Franklin Pugh, José E. Pérez-Ortín, Susana Rodríguez-Navarro

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Eukaryotic transcription is regulated through two complexes, the general transcription factor IID (TFIID) and the coactivator Spt-Ada-Gcn5 acetyltransferase (SAGA). Recent findings confirm that both TFIID and SAGA contribute to the synthesis of nearly all transcripts and are recruited genome-wide in yeast. However, how this broad recruitment confers selectivity under specific conditions remains an open question. Results: Here we find that the SAGA/TREX-2 subunit Sus1 associates with upstream regulatory regions of many yeast genes and that heat shock drastically changes Sus1 binding. While Sus1 binding to TFIID-dominated genes is not affected by temperature, its recruitment to SAGA-dominated genes and RP genes is significantly disturbed under heat shock, with Sus1 relocated to environmental stress-responsive genes in these conditions. Moreover, in contrast to recent results showing that SAGA deubiquitinating enzyme Ubp8 is dispensable for RNA synthesis, genomic run-on experiments demonstrate that Sus1 contributes to synthesis and stability of a wide range of transcripts. Conclusions: Our study provides support for a model in which SAGA/TREX-2 factor Sus1 acts as a global transcriptional regulator in yeast but has differential activity at yeast genes as a function of their transcription rate or during stress conditions.

Original languageEnglish (US)
Article number13
JournalEpigenetics and Chromatin
Volume11
Issue number1
DOIs
StatePublished - Mar 29 2018

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Acetyltransferases
Transcription Factor TFIID
Messenger RNA
Yeasts
Genes
Shock
Hot Temperature
General Transcription Factors
Nucleic Acid Regulatory Sequences
Genome
RNA
Temperature

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

García-Molinero, V., García-Martínez, J., Reja, R., Furió-Tarí, P., Antúnez, O., Vinayachandran, V., ... Rodríguez-Navarro, S. (2018). The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally. Epigenetics and Chromatin, 11(1), [13]. https://doi.org/10.1186/s13072-018-0184-2
García-Molinero, Varinia ; García-Martínez, José ; Reja, Rohit ; Furió-Tarí, Pedro ; Antúnez, Oreto ; Vinayachandran, Vinesh ; Conesa, Ana ; Pugh, B. Franklin ; Pérez-Ortín, José E. ; Rodríguez-Navarro, Susana. / The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally. In: Epigenetics and Chromatin. 2018 ; Vol. 11, No. 1.
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abstract = "Background: Eukaryotic transcription is regulated through two complexes, the general transcription factor IID (TFIID) and the coactivator Spt-Ada-Gcn5 acetyltransferase (SAGA). Recent findings confirm that both TFIID and SAGA contribute to the synthesis of nearly all transcripts and are recruited genome-wide in yeast. However, how this broad recruitment confers selectivity under specific conditions remains an open question. Results: Here we find that the SAGA/TREX-2 subunit Sus1 associates with upstream regulatory regions of many yeast genes and that heat shock drastically changes Sus1 binding. While Sus1 binding to TFIID-dominated genes is not affected by temperature, its recruitment to SAGA-dominated genes and RP genes is significantly disturbed under heat shock, with Sus1 relocated to environmental stress-responsive genes in these conditions. Moreover, in contrast to recent results showing that SAGA deubiquitinating enzyme Ubp8 is dispensable for RNA synthesis, genomic run-on experiments demonstrate that Sus1 contributes to synthesis and stability of a wide range of transcripts. Conclusions: Our study provides support for a model in which SAGA/TREX-2 factor Sus1 acts as a global transcriptional regulator in yeast but has differential activity at yeast genes as a function of their transcription rate or during stress conditions.",
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García-Molinero, V, García-Martínez, J, Reja, R, Furió-Tarí, P, Antúnez, O, Vinayachandran, V, Conesa, A, Pugh, BF, Pérez-Ortín, JE & Rodríguez-Navarro, S 2018, 'The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally', Epigenetics and Chromatin, vol. 11, no. 1, 13. https://doi.org/10.1186/s13072-018-0184-2

The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally. / García-Molinero, Varinia; García-Martínez, José; Reja, Rohit; Furió-Tarí, Pedro; Antúnez, Oreto; Vinayachandran, Vinesh; Conesa, Ana; Pugh, B. Franklin; Pérez-Ortín, José E.; Rodríguez-Navarro, Susana.

In: Epigenetics and Chromatin, Vol. 11, No. 1, 13, 29.03.2018.

Research output: Contribution to journalArticle

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AU - García-Molinero, Varinia

AU - García-Martínez, José

AU - Reja, Rohit

AU - Furió-Tarí, Pedro

AU - Antúnez, Oreto

AU - Vinayachandran, Vinesh

AU - Conesa, Ana

AU - Pugh, B. Franklin

AU - Pérez-Ortín, José E.

AU - Rodríguez-Navarro, Susana

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Y1 - 2018/3/29

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García-Molinero V, García-Martínez J, Reja R, Furió-Tarí P, Antúnez O, Vinayachandran V et al. The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally. Epigenetics and Chromatin. 2018 Mar 29;11(1). 13. https://doi.org/10.1186/s13072-018-0184-2