The sigA gene encoding the major σ factor of RNA polymerase from the marine cyanobacterium Synechococcus sp. strain PCC 7002: Cloning and characterization

Laurie F. Caslake, Donald A. Bryant

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20 Citations (Scopus)

Abstract

The gene encoding the principal σ factor from Synechococcus sp. strain PCC 7002 was isolated and characterized. The Synechococcus sp. strain PCC 7002 sigA gene encodes a protein of 375 amino acids (43.7 kDa) that is required for viability under normal growth conditions. The SigA protein was overproduced in Escherichia coil and the purified protein was used to raise polyclonal antiserum in rabbits. This antiserum was used in immunoblot analyses of partially purified RNA polymerase from Synechococcus sp. strain PR6000. The probable in vivo translational start site was identified by a comparison of amino acid sequencing results obtained with SigA proteins overproduced in E. coli with immunoblot analyses of SigA protein in crude preparations of RNA polymerase from the cyanobacterium. The sigA gene is encoded on a transcript of 1700 bases that initiates 496 nucleotides upstream from the probable in vivo translational start site. The abundance of sigA transcripts decreases rapidly after the removal of combined nitrogen from the growth medium.

Original languageEnglish (US)
Pages (from-to)347-357
Number of pages11
JournalMicrobiology
Volume142
Issue number2
DOIs
StatePublished - Feb 1996

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Synechococcus
Cyanobacteria
DNA-Directed RNA Polymerases
Organism Cloning
Genes
Proteins
Immune Sera
Escherichia
Protein Sequence Analysis
Growth
Nitrogen
Nucleotides
Escherichia coli
Rabbits
Amino Acids

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

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