Abstract
The gene encoding the principal σ factor from Synechococcus sp. strain PCC 7002 was isolated and characterized. The Synechococcus sp. strain PCC 7002 sigA gene encodes a protein of 375 amino acids (43.7 kDa) that is required for viability under normal growth conditions. The SigA protein was overproduced in Escherichia coil and the purified protein was used to raise polyclonal antiserum in rabbits. This antiserum was used in immunoblot analyses of partially purified RNA polymerase from Synechococcus sp. strain PR6000. The probable in vivo translational start site was identified by a comparison of amino acid sequencing results obtained with SigA proteins overproduced in E. coli with immunoblot analyses of SigA protein in crude preparations of RNA polymerase from the cyanobacterium. The sigA gene is encoded on a transcript of 1700 bases that initiates 496 nucleotides upstream from the probable in vivo translational start site. The abundance of sigA transcripts decreases rapidly after the removal of combined nitrogen from the growth medium.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 347-357 |
| Number of pages | 11 |
| Journal | Microbiology |
| Volume | 142 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1996 |
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All Science Journal Classification (ASJC) codes
- Microbiology
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The sigA gene encoding the major σ factor of RNA polymerase from the marine cyanobacterium Synechococcus sp. strain PCC 7002 : Cloning and characterization. / Caslake, Laurie F.; Bryant, Donald A.
In: Microbiology, Vol. 142, No. 2, 02.1996, p. 347-357.Research output: Contribution to journal › Article
TY - JOUR
T1 - The sigA gene encoding the major σ factor of RNA polymerase from the marine cyanobacterium Synechococcus sp. strain PCC 7002
T2 - Cloning and characterization
AU - Caslake, Laurie F.
AU - Bryant, Donald A.
PY - 1996/2
Y1 - 1996/2
N2 - The gene encoding the principal σ factor from Synechococcus sp. strain PCC 7002 was isolated and characterized. The Synechococcus sp. strain PCC 7002 sigA gene encodes a protein of 375 amino acids (43.7 kDa) that is required for viability under normal growth conditions. The SigA protein was overproduced in Escherichia coil and the purified protein was used to raise polyclonal antiserum in rabbits. This antiserum was used in immunoblot analyses of partially purified RNA polymerase from Synechococcus sp. strain PR6000. The probable in vivo translational start site was identified by a comparison of amino acid sequencing results obtained with SigA proteins overproduced in E. coli with immunoblot analyses of SigA protein in crude preparations of RNA polymerase from the cyanobacterium. The sigA gene is encoded on a transcript of 1700 bases that initiates 496 nucleotides upstream from the probable in vivo translational start site. The abundance of sigA transcripts decreases rapidly after the removal of combined nitrogen from the growth medium.
AB - The gene encoding the principal σ factor from Synechococcus sp. strain PCC 7002 was isolated and characterized. The Synechococcus sp. strain PCC 7002 sigA gene encodes a protein of 375 amino acids (43.7 kDa) that is required for viability under normal growth conditions. The SigA protein was overproduced in Escherichia coil and the purified protein was used to raise polyclonal antiserum in rabbits. This antiserum was used in immunoblot analyses of partially purified RNA polymerase from Synechococcus sp. strain PR6000. The probable in vivo translational start site was identified by a comparison of amino acid sequencing results obtained with SigA proteins overproduced in E. coli with immunoblot analyses of SigA protein in crude preparations of RNA polymerase from the cyanobacterium. The sigA gene is encoded on a transcript of 1700 bases that initiates 496 nucleotides upstream from the probable in vivo translational start site. The abundance of sigA transcripts decreases rapidly after the removal of combined nitrogen from the growth medium.
UR - http://www.scopus.com/inward/record.url?scp=0030025410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030025410&partnerID=8YFLogxK
U2 - 10.1099/13500872-142-2-347
DO - 10.1099/13500872-142-2-347
M3 - Article
C2 - 8932708
AN - SCOPUS:0030025410
VL - 142
SP - 347
EP - 357
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - 2
ER -