The skin, a novel niche for recirculating B cells

Skye A. Geherin, Sarah R. Fintushel, Michael H. Lee, R. Paul Wilson, Reema T. Patel, Carsten Alt, Alan J. Young, John B. Hay, Gudrun F. Debes

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

B cells infiltrate the skin in many chronic inflammatory diseases caused by autoimmunity or infection. Despite potential contribution to disease, skin-associated B cells remain poorly characterized. Using an ovine model of granulomatous skin inflammation, we demonstrate that B cells increase in the skin and skin-draining afferent lymph during inflammation. Surprisingly, skin B cells are a heterogeneous population that is distinct from lymph node B cells, with more large lymphocytes as well as B-1-like B cells that coexpress high levels of IgM and CD11b. Skin B cells have increased MHC class II, CD1, and CD80/86 expression compared with lymph node B cells, suggesting that they are well-suited for T cell activation at the site of inflammation. Furthermore, we show that skin accumulation of B cells and Ab-secreting cells during inflammation increases local Ab titers, which could augment host defense and autoimmunity. Although skin B cells express typical skin-homing receptors, such as E-selectin ligand and α-4 and β-1 integrins, they are unresponsive to ligands for chemokine receptors associated with T cell homing into skin. Instead, skin B cells migrate toward the cutaneously expressed CCR6 ligand CCL20. Our data support a model in which B cells use CCR6- CCL20 to recirculate through the skin, fulfilling a novel role in skin immunity and inflammation.

Original languageEnglish (US)
Pages (from-to)6027-6035
Number of pages9
JournalJournal of Immunology
Volume188
Issue number12
DOIs
StatePublished - Jun 15 2012

Fingerprint

B-Lymphocytes
Skin
Inflammation
Autoimmunity
Lymph Nodes
Ligands
T-Lymphocytes
Chemokine Receptors
Lymph
Skin Diseases
Integrins
Immunoglobulin M
Immunity
Sheep
Chronic Disease
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Geherin, S. A., Fintushel, S. R., Lee, M. H., Wilson, R. P., Patel, R. T., Alt, C., ... Debes, G. F. (2012). The skin, a novel niche for recirculating B cells. Journal of Immunology, 188(12), 6027-6035. https://doi.org/10.4049/jimmunol.1102639
Geherin, Skye A. ; Fintushel, Sarah R. ; Lee, Michael H. ; Wilson, R. Paul ; Patel, Reema T. ; Alt, Carsten ; Young, Alan J. ; Hay, John B. ; Debes, Gudrun F. / The skin, a novel niche for recirculating B cells. In: Journal of Immunology. 2012 ; Vol. 188, No. 12. pp. 6027-6035.
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Geherin, SA, Fintushel, SR, Lee, MH, Wilson, RP, Patel, RT, Alt, C, Young, AJ, Hay, JB & Debes, GF 2012, 'The skin, a novel niche for recirculating B cells', Journal of Immunology, vol. 188, no. 12, pp. 6027-6035. https://doi.org/10.4049/jimmunol.1102639

The skin, a novel niche for recirculating B cells. / Geherin, Skye A.; Fintushel, Sarah R.; Lee, Michael H.; Wilson, R. Paul; Patel, Reema T.; Alt, Carsten; Young, Alan J.; Hay, John B.; Debes, Gudrun F.

In: Journal of Immunology, Vol. 188, No. 12, 15.06.2012, p. 6027-6035.

Research output: Contribution to journalArticle

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Geherin SA, Fintushel SR, Lee MH, Wilson RP, Patel RT, Alt C et al. The skin, a novel niche for recirculating B cells. Journal of Immunology. 2012 Jun 15;188(12):6027-6035. https://doi.org/10.4049/jimmunol.1102639