The small GTPase RSG1 controls a final step in primary cilia initiation

Stephanie O. Agbu, Yinwen Liang, Aimin Liu, Kathryn V. Anderson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Primary cilia, which are essential for normal development and tissue homeostasis, are extensions of the mother centriole, but the mechanisms that remodel the centriole to promote cilia initiation are poorly understood. Here we show that mouse embryos that lack the small guanosine triphosphatase RSG1 die at embryonic day 12.5, with developmental abnormalities characteristic of decreased cilia-dependent Hedgehog signaling. Rsg1 mutant embryos have fewer primary cilia than wild-type embryos, but the cilia that form are of normal length and traffic Hedgehog pathway proteins within the cilium correctly. Rsg1 mother centrioles recruit proteins required for cilia initiation and dock onto ciliary vesicles, but axonemal microtubules fail to elongate normally. RSG1 localizes to the mother centriole in a process that depends on tau tubulin kinase 2 (TTBK2), the CPL ANE complex protein Inturned (INTU), and its own GTPase activity. The data suggest a specific role for RSG1 in the final maturation of the mother centriole and ciliary vesicle that allows extension of the ciliary axoneme.

Original languageEnglish (US)
Pages (from-to)413-427
Number of pages15
JournalJournal of Cell Biology
Volume217
Issue number1
DOIs
StatePublished - Jan 1 2018

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Monomeric GTP-Binding Proteins
Cilia
Centrioles
Embryonic Structures
Hedgehog Proteins
Axoneme
Hedgehogs
Guanosine
GTP Phosphohydrolases
Microtubules
Proteins
Homeostasis

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Agbu, Stephanie O. ; Liang, Yinwen ; Liu, Aimin ; Anderson, Kathryn V. / The small GTPase RSG1 controls a final step in primary cilia initiation. In: Journal of Cell Biology. 2018 ; Vol. 217, No. 1. pp. 413-427.
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The small GTPase RSG1 controls a final step in primary cilia initiation. / Agbu, Stephanie O.; Liang, Yinwen; Liu, Aimin; Anderson, Kathryn V.

In: Journal of Cell Biology, Vol. 217, No. 1, 01.01.2018, p. 413-427.

Research output: Contribution to journalArticle

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