The stimulating effects of ethanol on ventral tegmental area dopamine neurons projecting to the ventral pallidum and medial prefrontal cortex in female Wistar rats

Regional difference and involvement of serotonin-3 receptors

Zheng-Ming Ding, Scott M. Oster, Sarah R. Hall, Eric A. Engleman, Sheketha R. Hauser, William J. McBride, Zachary A. Rodd

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Rationale: The ventral tegmental area (VTA) mediates the local stimulating effects of ethanol (EtOH) in a region-dependent manner, with EtOH administration in the posterior but not anterior VTA stimulating the mesolimbic system. The serotonin-3 (5-HT3) receptor has been involved in the effects of EtOH on the mesolimbic system. Objectives: The current study tested the hypothesis that EtOH would stimulate mesopallidal and mesocortical dopamine neurons in the posterior but not anterior VTA and that the stimulating effects of EtOH in the VTA would involve activation of local 5-HT3 receptors. Methods: Wistar female rats were surgically implanted with two cannulae, one in one sub-region of the VTA for microinjection and the other in the ventral pallidum (VP) or medial prefrontal cortex (mPFC) for microdialysis. Artificial cerebrospinal fluid or EtOH (200 mg%; 44 mM) was microinjected in the anterior or posterior VTA, and extracellular dopamine was measured in the VP or mPFC with microdialysis-HPLC. Results: EtOH injections in the posterior but not anterior VTA significantly increased extracellular dopamine levels in the VP and mPFC. Co-injections of the 5-HT3 receptor antagonist ICS-205,930 with EtOH in the posterior VTA significantly reduced the effects of EtOH on extracellular dopamine levels in the VP and mPFC. Conclusions: The results indicate that posterior VTA dopamine neurons projecting to the VP and mPFC are stimulated by local administration of EtOH and that the local stimulating effects of EtOH are mediated, at least in part, by 5-HT3 receptors.

Original languageEnglish (US)
Pages (from-to)245-255
Number of pages11
JournalPsychopharmacology
Volume216
Issue number2
DOIs
StatePublished - Jul 1 2011

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Receptors, Serotonin, 5-HT3
Ventral Tegmental Area
Dopaminergic Neurons
Prefrontal Cortex
Wistar Rats
Ethanol
Dopamine
tropisetron
Microdialysis
Serotonin 5-HT3 Receptor Antagonists
Basal Forebrain
Injections
Microinjections
Cerebrospinal Fluid
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

@article{e6f1e0b459b5448ebdc2a539183a1f56,
title = "The stimulating effects of ethanol on ventral tegmental area dopamine neurons projecting to the ventral pallidum and medial prefrontal cortex in female Wistar rats: Regional difference and involvement of serotonin-3 receptors",
abstract = "Rationale: The ventral tegmental area (VTA) mediates the local stimulating effects of ethanol (EtOH) in a region-dependent manner, with EtOH administration in the posterior but not anterior VTA stimulating the mesolimbic system. The serotonin-3 (5-HT3) receptor has been involved in the effects of EtOH on the mesolimbic system. Objectives: The current study tested the hypothesis that EtOH would stimulate mesopallidal and mesocortical dopamine neurons in the posterior but not anterior VTA and that the stimulating effects of EtOH in the VTA would involve activation of local 5-HT3 receptors. Methods: Wistar female rats were surgically implanted with two cannulae, one in one sub-region of the VTA for microinjection and the other in the ventral pallidum (VP) or medial prefrontal cortex (mPFC) for microdialysis. Artificial cerebrospinal fluid or EtOH (200 mg{\%}; 44 mM) was microinjected in the anterior or posterior VTA, and extracellular dopamine was measured in the VP or mPFC with microdialysis-HPLC. Results: EtOH injections in the posterior but not anterior VTA significantly increased extracellular dopamine levels in the VP and mPFC. Co-injections of the 5-HT3 receptor antagonist ICS-205,930 with EtOH in the posterior VTA significantly reduced the effects of EtOH on extracellular dopamine levels in the VP and mPFC. Conclusions: The results indicate that posterior VTA dopamine neurons projecting to the VP and mPFC are stimulated by local administration of EtOH and that the local stimulating effects of EtOH are mediated, at least in part, by 5-HT3 receptors.",
author = "Zheng-Ming Ding and Oster, {Scott M.} and Hall, {Sarah R.} and Engleman, {Eric A.} and Hauser, {Sheketha R.} and McBride, {William J.} and Rodd, {Zachary A.}",
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The stimulating effects of ethanol on ventral tegmental area dopamine neurons projecting to the ventral pallidum and medial prefrontal cortex in female Wistar rats : Regional difference and involvement of serotonin-3 receptors. / Ding, Zheng-Ming; Oster, Scott M.; Hall, Sarah R.; Engleman, Eric A.; Hauser, Sheketha R.; McBride, William J.; Rodd, Zachary A.

In: Psychopharmacology, Vol. 216, No. 2, 01.07.2011, p. 245-255.

Research output: Contribution to journalArticle

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T1 - The stimulating effects of ethanol on ventral tegmental area dopamine neurons projecting to the ventral pallidum and medial prefrontal cortex in female Wistar rats

T2 - Regional difference and involvement of serotonin-3 receptors

AU - Ding, Zheng-Ming

AU - Oster, Scott M.

AU - Hall, Sarah R.

AU - Engleman, Eric A.

AU - Hauser, Sheketha R.

AU - McBride, William J.

AU - Rodd, Zachary A.

PY - 2011/7/1

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N2 - Rationale: The ventral tegmental area (VTA) mediates the local stimulating effects of ethanol (EtOH) in a region-dependent manner, with EtOH administration in the posterior but not anterior VTA stimulating the mesolimbic system. The serotonin-3 (5-HT3) receptor has been involved in the effects of EtOH on the mesolimbic system. Objectives: The current study tested the hypothesis that EtOH would stimulate mesopallidal and mesocortical dopamine neurons in the posterior but not anterior VTA and that the stimulating effects of EtOH in the VTA would involve activation of local 5-HT3 receptors. Methods: Wistar female rats were surgically implanted with two cannulae, one in one sub-region of the VTA for microinjection and the other in the ventral pallidum (VP) or medial prefrontal cortex (mPFC) for microdialysis. Artificial cerebrospinal fluid or EtOH (200 mg%; 44 mM) was microinjected in the anterior or posterior VTA, and extracellular dopamine was measured in the VP or mPFC with microdialysis-HPLC. Results: EtOH injections in the posterior but not anterior VTA significantly increased extracellular dopamine levels in the VP and mPFC. Co-injections of the 5-HT3 receptor antagonist ICS-205,930 with EtOH in the posterior VTA significantly reduced the effects of EtOH on extracellular dopamine levels in the VP and mPFC. Conclusions: The results indicate that posterior VTA dopamine neurons projecting to the VP and mPFC are stimulated by local administration of EtOH and that the local stimulating effects of EtOH are mediated, at least in part, by 5-HT3 receptors.

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