The structure of cyanobacterial phycobilisomes

a model

Donald Ashley Bryant, Gérard Guglielmi, Nicole Tandeau de Marsac, Anne Marie Castets, Germaine Cohen-Bazire

Research output: Contribution to journalArticle

201 Citations (Scopus)

Abstract

Phycobilisomes, supramolecular complexes of water-soluble accessory pigments, serve as the major light-harvesting antennae in cyanobacteria and red algae. Regular arrays of these organelles are found on the surface of the thylakoid membranes of these organisms. In the present study, the hemi-discoidal phycobilisomes of several species of cyanobacteria were examined in thin sections of cells and by negative staining after isolation and fixation. Their fundamental structures were found to be the same. Isolated phycobilisomes possessed a triangular core assembled from three stacks of disc-shaped subunits. Each stack contained two discs which were ∼12 nm in diameter and ∼6-7 nm thick. Each of these discs was probably subdivided into halves ∼3-3.5 nm thick. Radiating from each of two sides of the triangular core were three rods ∼12 nm in diameter. Each rod consisted of stacks of 2 to 6 disc-shaped subunits ∼6 nm thick. These discs were subdivided into halves ∼3 nm thick. The average number of discs of ∼6 nm thickness forming the peripheral rods varied among the strains studied. For certain chromatically adapting strains, the average rod length was dependent upon the wavelength of light to which cells were exposed during growth. Analyses of phycobilisomes by spectroscopic techniques, polyacrylamide gel electrophoresis, and electron microscopy were compared. These analyses suggested that the triangular core was composed of allophycocyanin and that the peripheral rods contained phycocyanin and phycoerythrin (when present). A detailed model of the hemi-discoidal phycobilisome is proposed. This model can account for many aspects of phycobiliprotein assembly and energy transfer.

Original languageEnglish (US)
Pages (from-to)113-127
Number of pages15
JournalArchives of Microbiology
Volume123
Issue number2
DOIs
StatePublished - Nov 1 1979

Fingerprint

Phycobilisomes
Phycobiliproteins
Phycocyanin
Phycoerythrin
Accessories
Algae
Electrophoresis
Pigments
Energy transfer
Electron microscopy
Cyanobacteria
Antennas
Membranes
Wavelength
Water
Rhodophyta
Light
Negative Staining
Thylakoids
Energy Transfer

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Bryant, D. A., Guglielmi, G., de Marsac, N. T., Castets, A. M., & Cohen-Bazire, G. (1979). The structure of cyanobacterial phycobilisomes: a model. Archives of Microbiology, 123(2), 113-127. https://doi.org/10.1007/BF00446810
Bryant, Donald Ashley ; Guglielmi, Gérard ; de Marsac, Nicole Tandeau ; Castets, Anne Marie ; Cohen-Bazire, Germaine. / The structure of cyanobacterial phycobilisomes : a model. In: Archives of Microbiology. 1979 ; Vol. 123, No. 2. pp. 113-127.
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Bryant, DA, Guglielmi, G, de Marsac, NT, Castets, AM & Cohen-Bazire, G 1979, 'The structure of cyanobacterial phycobilisomes: a model', Archives of Microbiology, vol. 123, no. 2, pp. 113-127. https://doi.org/10.1007/BF00446810

The structure of cyanobacterial phycobilisomes : a model. / Bryant, Donald Ashley; Guglielmi, Gérard; de Marsac, Nicole Tandeau; Castets, Anne Marie; Cohen-Bazire, Germaine.

In: Archives of Microbiology, Vol. 123, No. 2, 01.11.1979, p. 113-127.

Research output: Contribution to journalArticle

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N2 - Phycobilisomes, supramolecular complexes of water-soluble accessory pigments, serve as the major light-harvesting antennae in cyanobacteria and red algae. Regular arrays of these organelles are found on the surface of the thylakoid membranes of these organisms. In the present study, the hemi-discoidal phycobilisomes of several species of cyanobacteria were examined in thin sections of cells and by negative staining after isolation and fixation. Their fundamental structures were found to be the same. Isolated phycobilisomes possessed a triangular core assembled from three stacks of disc-shaped subunits. Each stack contained two discs which were ∼12 nm in diameter and ∼6-7 nm thick. Each of these discs was probably subdivided into halves ∼3-3.5 nm thick. Radiating from each of two sides of the triangular core were three rods ∼12 nm in diameter. Each rod consisted of stacks of 2 to 6 disc-shaped subunits ∼6 nm thick. These discs were subdivided into halves ∼3 nm thick. The average number of discs of ∼6 nm thickness forming the peripheral rods varied among the strains studied. For certain chromatically adapting strains, the average rod length was dependent upon the wavelength of light to which cells were exposed during growth. Analyses of phycobilisomes by spectroscopic techniques, polyacrylamide gel electrophoresis, and electron microscopy were compared. These analyses suggested that the triangular core was composed of allophycocyanin and that the peripheral rods contained phycocyanin and phycoerythrin (when present). A detailed model of the hemi-discoidal phycobilisome is proposed. This model can account for many aspects of phycobiliprotein assembly and energy transfer.

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Bryant DA, Guglielmi G, de Marsac NT, Castets AM, Cohen-Bazire G. The structure of cyanobacterial phycobilisomes: a model. Archives of Microbiology. 1979 Nov 1;123(2):113-127. https://doi.org/10.1007/BF00446810