The Structure of the S127P Mutant of Cytochrome b5 Reductase that Causes Methemoglobinemia Shows the AMP Moiety of the Flavin Occupying the Substrate Binding Site

Maria Bewley, C. Ainsley Davis, Christopher C. Marohnic, David Taormina, Michael J. Barber

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Abstract

Methemoglobinemia, the first hereditary disease to be identified that involved an enzyme deficiency, has been ascribed to mutations in the enzyme cytochrome b5 reductase. A variety of defects in either the erythrocytic or microsomal forms of the enzyme have been identified that give rise to the type I or type II variant of the disease, respectively. The positions of the methemoglobinemia-causing mutations are scattered throughout the protein sequence, but the majority of the nontruncated mutants that produce type II symptoms occur close to the flavin adenine dinucleotide (FAD) cofactor binding site. While X-ray structures have been determined for the soluble, flavin-containing diaphorase domains of the rat and pig enzymes, no X-ray or NMR structure has been described for the human enzyme or any of the methemoglobinemia variants. S127P, a mutant that causes type II methemoglobinemia, was the first to be positively identified and have its spectroscopic and kinetic properties characterized that revealed altered nicotinamide adenine dinucleotide hydride (NADH) substrate binding behavior. To understand these changes at a structural level, we have determined the structure of the S127P mutant of rat cytochrome b5 reductase to 1.8 Å resolution, providing the first structural snapshot of a cytochrome b5 reductase mutant that causes methemoglobinemia. The high-resolution structure revealed that the adenosine diphosphate (ADP) moiety of the FAD prosthetic group is displaced into the corresponding ADP binding site of the physiological substrate, NADH, thus acting as a substrate inhibitor which is consistent with both the spectroscopic and kinetic data.

Original languageEnglish (US)
Pages (from-to)13145-13151
Number of pages7
JournalBiochemistry
Volume42
Issue number45
DOIs
Publication statusPublished - Nov 18 2003

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All Science Journal Classification (ASJC) codes

  • Biochemistry

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