The TCN2 776C>G polymorphism correlates with vitamin B12 cellular delivery in healthy adult populations

Rita Castro, Madalena Barroso, Mónica Rocha, Ruben Esse, Ruben Ramos, Paula Ravasco, Isabel Rivera, Isabel Tavares de Almeida

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objectives: Vitamin B12, or B12, is an essential nutrient for humans, and its deficiency is a public health problem, especially in elderly population. Around 30% of circulating total B12 levels are attached to transcobalamin II (TCN2), being referred as holotranscobalamin (holo-TC), and representing the biologically active fraction. After cellular uptake, B12 participates in the homocysteine (Hcy) metabolism. The potential influence of the described TCN2 776C>G polymorphism upon B12 intracellular delivery is a current target of research and we aimed to investigate its biochemical significance upon a healthy adult population. Design and methods: The TCN2 776C>G polymorphism was screened by PCR-RFLP in 122 individuals. Concentrations of plasma total B12, holo-TC, total Hcy and folate, as well as red blood cell folate, were determined. Results and conclusions: The studied polymorphism is common in the Portuguese population and significantly affects holo-TC but neither total B12 nor total Hcy plasma concentrations, confirming that the TCN2 776C>G genotype exerts a significant influence upon B12 cellular delivery.

Original languageEnglish (US)
Pages (from-to)645-649
Number of pages5
JournalClinical Biochemistry
Volume43
Issue number7-8
DOIs
StatePublished - May 1 2010

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Homocysteine
Vitamin B 12
Polymorphism
Folic Acid
Transcobalamins
Population
Plasmas
Public health
Medical problems
Metabolism
Restriction Fragment Length Polymorphisms
Nutrients
Blood
Public Health
Erythrocytes
Genotype
Cells
Food
Polymerase Chain Reaction
Research

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Castro, Rita ; Barroso, Madalena ; Rocha, Mónica ; Esse, Ruben ; Ramos, Ruben ; Ravasco, Paula ; Rivera, Isabel ; de Almeida, Isabel Tavares. / The TCN2 776C>G polymorphism correlates with vitamin B12 cellular delivery in healthy adult populations. In: Clinical Biochemistry. 2010 ; Vol. 43, No. 7-8. pp. 645-649.
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Castro, R, Barroso, M, Rocha, M, Esse, R, Ramos, R, Ravasco, P, Rivera, I & de Almeida, IT 2010, 'The TCN2 776C>G polymorphism correlates with vitamin B12 cellular delivery in healthy adult populations', Clinical Biochemistry, vol. 43, no. 7-8, pp. 645-649. https://doi.org/10.1016/j.clinbiochem.2010.01.015

The TCN2 776C>G polymorphism correlates with vitamin B12 cellular delivery in healthy adult populations. / Castro, Rita; Barroso, Madalena; Rocha, Mónica; Esse, Ruben; Ramos, Ruben; Ravasco, Paula; Rivera, Isabel; de Almeida, Isabel Tavares.

In: Clinical Biochemistry, Vol. 43, No. 7-8, 01.05.2010, p. 645-649.

Research output: Contribution to journalArticle

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T1 - The TCN2 776C>G polymorphism correlates with vitamin B12 cellular delivery in healthy adult populations

AU - Castro, Rita

AU - Barroso, Madalena

AU - Rocha, Mónica

AU - Esse, Ruben

AU - Ramos, Ruben

AU - Ravasco, Paula

AU - Rivera, Isabel

AU - de Almeida, Isabel Tavares

PY - 2010/5/1

Y1 - 2010/5/1

N2 - Objectives: Vitamin B12, or B12, is an essential nutrient for humans, and its deficiency is a public health problem, especially in elderly population. Around 30% of circulating total B12 levels are attached to transcobalamin II (TCN2), being referred as holotranscobalamin (holo-TC), and representing the biologically active fraction. After cellular uptake, B12 participates in the homocysteine (Hcy) metabolism. The potential influence of the described TCN2 776C>G polymorphism upon B12 intracellular delivery is a current target of research and we aimed to investigate its biochemical significance upon a healthy adult population. Design and methods: The TCN2 776C>G polymorphism was screened by PCR-RFLP in 122 individuals. Concentrations of plasma total B12, holo-TC, total Hcy and folate, as well as red blood cell folate, were determined. Results and conclusions: The studied polymorphism is common in the Portuguese population and significantly affects holo-TC but neither total B12 nor total Hcy plasma concentrations, confirming that the TCN2 776C>G genotype exerts a significant influence upon B12 cellular delivery.

AB - Objectives: Vitamin B12, or B12, is an essential nutrient for humans, and its deficiency is a public health problem, especially in elderly population. Around 30% of circulating total B12 levels are attached to transcobalamin II (TCN2), being referred as holotranscobalamin (holo-TC), and representing the biologically active fraction. After cellular uptake, B12 participates in the homocysteine (Hcy) metabolism. The potential influence of the described TCN2 776C>G polymorphism upon B12 intracellular delivery is a current target of research and we aimed to investigate its biochemical significance upon a healthy adult population. Design and methods: The TCN2 776C>G polymorphism was screened by PCR-RFLP in 122 individuals. Concentrations of plasma total B12, holo-TC, total Hcy and folate, as well as red blood cell folate, were determined. Results and conclusions: The studied polymorphism is common in the Portuguese population and significantly affects holo-TC but neither total B12 nor total Hcy plasma concentrations, confirming that the TCN2 776C>G genotype exerts a significant influence upon B12 cellular delivery.

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