The Thermus thermophilus 5S rRNA-protein complex: Identification of specific binding sites for proteins L5 and L18 in the 5S rRNA

G. M. Gongadze, A. A. Perederina, V. A. Meshcheryakov, R. V. Fedorov, S. E. Moskalenko, A. V. Rak, A. A. Serganov, D. V. Shcherbakov, S. V. Nikonov, M. B. Garber

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Three 5S rRNA-binding ribosomal proteins (L5, L18, TL5) of extremely thermophilic bacterium Thermus thermophilus have earlier been isolated. Structural analysis of their complexes with rRNA requires identification of their binding sites in the 5S rRNA. Previously, a TL5-binding site has been identified, a TL5-RNA complex crystallized, and its structure determined to 2.3 Å. The sites for L5 and L18 were characterized, and two corresponding 5S rRNA fragments constructed. Of these, a 34-nt fragment specifically interacted with L5, and a 55-nt fragment interacted with L5, L18, and with both proteins. The 34-nt fragment-L5 complex was crystallized; the crystals are suitable for high-resolution X-ray analysis.

Original languageEnglish (US)
Pages (from-to)521-526
Number of pages6
JournalMolecular Biology
Volume35
Issue number4
DOIs
StatePublished - Jul 2001

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology

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