The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed

Xia Chen, Joanne E. Pfeil, Susannah Gal

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Genomic sequencing has identified three different typical plant aspartic proteinases in the genome of Arabidopsis thaliana, named Pasp-A1, A2 and A3. A1 is identical to a cDNA we had previously isolated and the two others produce proteins 81 and 63% identical to that predicted protein. Sequencing of the aspartic proteinase protein purified from Arabidopsis seeds showed that the peptides are derived from two of these genes, A1 and A2. Using gene specific probes, we have analyzed RNA from different tissues and found these three genes are differentially expressed. A1 mRNA is detected in all tissues analyzed and more abundant in leaves during the light phase of growth. The other two genes are expressed either primarily in flowers (A3) or in seeds (A2). In situ hybridization demonstrated that all three genes are expressed in many cells of the seeds and developing seed pods. The A1 and A3 genes are expressed in the sepals and petals of flowers as well as the outer layer of the style, but are not expressed in the transmitting tract or on the stigmatal surface. The A2 gene is weakly expressed only in the transmitting tissue of the style. All three genes are also expressed in the guard cells of sepals. These data suggest multiple roles for aspartic proteinases besides those proposed in seeds.

Original languageEnglish (US)
Pages (from-to)4675-4684
Number of pages10
JournalEuropean Journal of Biochemistry
Volume269
Issue number18
DOIs
StatePublished - Oct 9 2002

Fingerprint

Aspartic Acid Proteases
Arabidopsis
Genes
Seeds
varespladib methyl
Seed
Tissue
Arabidopsis Proteins
Proteins
In Situ Hybridization
Complementary DNA
Genome
RNA
Light
Messenger RNA
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

@article{44fb07704e9f4db6877a767097ae2ef7,
title = "The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed",
abstract = "Genomic sequencing has identified three different typical plant aspartic proteinases in the genome of Arabidopsis thaliana, named Pasp-A1, A2 and A3. A1 is identical to a cDNA we had previously isolated and the two others produce proteins 81 and 63{\%} identical to that predicted protein. Sequencing of the aspartic proteinase protein purified from Arabidopsis seeds showed that the peptides are derived from two of these genes, A1 and A2. Using gene specific probes, we have analyzed RNA from different tissues and found these three genes are differentially expressed. A1 mRNA is detected in all tissues analyzed and more abundant in leaves during the light phase of growth. The other two genes are expressed either primarily in flowers (A3) or in seeds (A2). In situ hybridization demonstrated that all three genes are expressed in many cells of the seeds and developing seed pods. The A1 and A3 genes are expressed in the sepals and petals of flowers as well as the outer layer of the style, but are not expressed in the transmitting tract or on the stigmatal surface. The A2 gene is weakly expressed only in the transmitting tissue of the style. All three genes are also expressed in the guard cells of sepals. These data suggest multiple roles for aspartic proteinases besides those proposed in seeds.",
author = "Xia Chen and Pfeil, {Joanne E.} and Susannah Gal",
year = "2002",
month = "10",
day = "9",
doi = "10.1046/j.1432-1033.2002.03168.x",
language = "English (US)",
volume = "269",
pages = "4675--4684",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "18",

}

The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed. / Chen, Xia; Pfeil, Joanne E.; Gal, Susannah.

In: European Journal of Biochemistry, Vol. 269, No. 18, 09.10.2002, p. 4675-4684.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed

AU - Chen, Xia

AU - Pfeil, Joanne E.

AU - Gal, Susannah

PY - 2002/10/9

Y1 - 2002/10/9

N2 - Genomic sequencing has identified three different typical plant aspartic proteinases in the genome of Arabidopsis thaliana, named Pasp-A1, A2 and A3. A1 is identical to a cDNA we had previously isolated and the two others produce proteins 81 and 63% identical to that predicted protein. Sequencing of the aspartic proteinase protein purified from Arabidopsis seeds showed that the peptides are derived from two of these genes, A1 and A2. Using gene specific probes, we have analyzed RNA from different tissues and found these three genes are differentially expressed. A1 mRNA is detected in all tissues analyzed and more abundant in leaves during the light phase of growth. The other two genes are expressed either primarily in flowers (A3) or in seeds (A2). In situ hybridization demonstrated that all three genes are expressed in many cells of the seeds and developing seed pods. The A1 and A3 genes are expressed in the sepals and petals of flowers as well as the outer layer of the style, but are not expressed in the transmitting tract or on the stigmatal surface. The A2 gene is weakly expressed only in the transmitting tissue of the style. All three genes are also expressed in the guard cells of sepals. These data suggest multiple roles for aspartic proteinases besides those proposed in seeds.

AB - Genomic sequencing has identified three different typical plant aspartic proteinases in the genome of Arabidopsis thaliana, named Pasp-A1, A2 and A3. A1 is identical to a cDNA we had previously isolated and the two others produce proteins 81 and 63% identical to that predicted protein. Sequencing of the aspartic proteinase protein purified from Arabidopsis seeds showed that the peptides are derived from two of these genes, A1 and A2. Using gene specific probes, we have analyzed RNA from different tissues and found these three genes are differentially expressed. A1 mRNA is detected in all tissues analyzed and more abundant in leaves during the light phase of growth. The other two genes are expressed either primarily in flowers (A3) or in seeds (A2). In situ hybridization demonstrated that all three genes are expressed in many cells of the seeds and developing seed pods. The A1 and A3 genes are expressed in the sepals and petals of flowers as well as the outer layer of the style, but are not expressed in the transmitting tract or on the stigmatal surface. The A2 gene is weakly expressed only in the transmitting tissue of the style. All three genes are also expressed in the guard cells of sepals. These data suggest multiple roles for aspartic proteinases besides those proposed in seeds.

UR - http://www.scopus.com/inward/record.url?scp=0036380769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036380769&partnerID=8YFLogxK

U2 - 10.1046/j.1432-1033.2002.03168.x

DO - 10.1046/j.1432-1033.2002.03168.x

M3 - Article

C2 - 12230581

AN - SCOPUS:0036380769

VL - 269

SP - 4675

EP - 4684

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 18

ER -