The transformylase enzymes of de novo purine biosynthesis

Mark S. Warren, Karen M. Mattia, Ariane E. Marolewski, Stephen Benkovic

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Formyl transfer reactions play a key role in the construction of the purine heterocycle during de novo purine biosynthesis. Formylation is catalyzed early in the pathway by the purN glycinamide ribonucleotide transformylase (GAR Transformylase, EC 2.1.2.2) in a tetrahydrofolate-dependent manner and also by the purT GAR transformylase in a tetrahydrofolate-independent manner in bacteria. Late in the pathway, 5-aminoimidazole-4-carboxamide ribonucleotide transformylase (AICAR Transformylase, EC 2.1.2.3) catalyzes the second and final formylation involved in purine nucleotide biosynthesis. This article summarizes the salient properties and mechanistic knowledge on the transformylases with special emphasis on the mechanism of the purN GAR transformylase as explored by mutagenesis studies.

Original languageEnglish (US)
Pages (from-to)2029-2036
Number of pages8
JournalPure and Applied Chemistry
Volume68
Issue number11
DOIs
StatePublished - Jan 1 1996

Fingerprint

Phosphoribosylglycinamide Formyltransferase
Hydroxymethyl and Formyl Transferases
Biosynthesis
Enzymes
Mutagenesis
Nucleotides
Phosphoribosylaminoimidazolecarboxamide Formyltransferase
Bacteria
Purine Nucleotides
Ribonucleotides
purine

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Warren, M. S., Mattia, K. M., Marolewski, A. E., & Benkovic, S. (1996). The transformylase enzymes of de novo purine biosynthesis. Pure and Applied Chemistry, 68(11), 2029-2036. https://doi.org/10.1351/pac199668112029
Warren, Mark S. ; Mattia, Karen M. ; Marolewski, Ariane E. ; Benkovic, Stephen. / The transformylase enzymes of de novo purine biosynthesis. In: Pure and Applied Chemistry. 1996 ; Vol. 68, No. 11. pp. 2029-2036.
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Warren, MS, Mattia, KM, Marolewski, AE & Benkovic, S 1996, 'The transformylase enzymes of de novo purine biosynthesis', Pure and Applied Chemistry, vol. 68, no. 11, pp. 2029-2036. https://doi.org/10.1351/pac199668112029

The transformylase enzymes of de novo purine biosynthesis. / Warren, Mark S.; Mattia, Karen M.; Marolewski, Ariane E.; Benkovic, Stephen.

In: Pure and Applied Chemistry, Vol. 68, No. 11, 01.01.1996, p. 2029-2036.

Research output: Contribution to journalArticle

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Warren MS, Mattia KM, Marolewski AE, Benkovic S. The transformylase enzymes of de novo purine biosynthesis. Pure and Applied Chemistry. 1996 Jan 1;68(11):2029-2036. https://doi.org/10.1351/pac199668112029