The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes

Gary H. Perdew; Henry W. Schaup; Daniel P. Selivonchick

doi:10.1016/0003-2697(83)90711-X

The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes

Gary H. Perdew, Henry W. Schaup, Daniel P. Selivonchick

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96 Scopus citations

Abstract

CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-Propane sulfonate, a zwitterionic detergent, has been shown to exhibit superior membrane protein solubilizing characteristics as compared to nonionic detergents. Replacement of NP-40 with CHAPS in isoelectric focusing of rainbow trout liver microsomes has increased resolution markedly. The two-dimensional electrophoretic system described will allow effective resolution of up to 300 μg of crude microsomal protein. CHAPS exhibits no effect on the stability or type of pH gradient when compared to NP-40 during isoelectric focusing in the presence of urea.

Original language	English (US)
Pages (from-to)	453-455
Number of pages	3
Journal	Analytical Biochemistry
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(83)90711-X
State	Published - Dec 1983

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All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Perdew, G. H., Schaup, H. W., & Selivonchick, D. P. (1983). The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes. Analytical Biochemistry, 135(2), 453-455. https://doi.org/10.1016/0003-2697(83)90711-X

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AU - Selivonchick, Daniel P.

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AB - CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-Propane sulfonate, a zwitterionic detergent, has been shown to exhibit superior membrane protein solubilizing characteristics as compared to nonionic detergents. Replacement of NP-40 with CHAPS in isoelectric focusing of rainbow trout liver microsomes has increased resolution markedly. The two-dimensional electrophoretic system described will allow effective resolution of up to 300 μg of crude microsomal protein. CHAPS exhibits no effect on the stability or type of pH gradient when compared to NP-40 during isoelectric focusing in the presence of urea.

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10.1016/0003-2697(83)90711-X