The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes

Gary H. Perdew; Henry W. Schaup; Daniel P. Selivonchick

doi:10.1016/0003-2697(83)90711-X

The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes

Gary H. Perdew, Henry W. Schaup, Daniel P. Selivonchick

Research output: Contribution to journal › Article › peer-review

99 Scopus citations

Abstract

CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-Propane sulfonate, a zwitterionic detergent, has been shown to exhibit superior membrane protein solubilizing characteristics as compared to nonionic detergents. Replacement of NP-40 with CHAPS in isoelectric focusing of rainbow trout liver microsomes has increased resolution markedly. The two-dimensional electrophoretic system described will allow effective resolution of up to 300 μg of crude microsomal protein. CHAPS exhibits no effect on the stability or type of pH gradient when compared to NP-40 during isoelectric focusing in the presence of urea.

Original language	English (US)
Pages (from-to)	453-455
Number of pages	3
Journal	Analytical Biochemistry
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(83)90711-X
State	Published - Dec 1983

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(83)90711-X

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title = "The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes",

abstract = "CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-Propane sulfonate, a zwitterionic detergent, has been shown to exhibit superior membrane protein solubilizing characteristics as compared to nonionic detergents. Replacement of NP-40 with CHAPS in isoelectric focusing of rainbow trout liver microsomes has increased resolution markedly. The two-dimensional electrophoretic system described will allow effective resolution of up to 300 μg of crude microsomal protein. CHAPS exhibits no effect on the stability or type of pH gradient when compared to NP-40 during isoelectric focusing in the presence of urea.",

author = "Perdew, {Gary H.} and Schaup, {Henry W.} and Selivonchick, {Daniel P.}",

note = "Funding Information: The establishment of two-dimensional gel electrophoresis techniques in our laboratory by Mark Einerson is gratefully acknowledged This investigation was supported by Grant 1ROlCA 30087-03 from the United States Public Health Service. Technical Paper No. 68 11, Oregon Agricultural Experiment Station, Oregon State University, Corvallis, Oregon 9733 1.",

year = "1983",

month = dec,

doi = "10.1016/0003-2697(83)90711-X",

language = "English (US)",

volume = "135",

pages = "453--455",

journal = "Analytical Biochemistry",

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T1 - The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes

AU - Perdew, Gary H.

AU - Schaup, Henry W.

AU - Selivonchick, Daniel P.

N1 - Funding Information: The establishment of two-dimensional gel electrophoresis techniques in our laboratory by Mark Einerson is gratefully acknowledged This investigation was supported by Grant 1ROlCA 30087-03 from the United States Public Health Service. Technical Paper No. 68 11, Oregon Agricultural Experiment Station, Oregon State University, Corvallis, Oregon 9733 1.

PY - 1983/12

Y1 - 1983/12

N2 - CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-Propane sulfonate, a zwitterionic detergent, has been shown to exhibit superior membrane protein solubilizing characteristics as compared to nonionic detergents. Replacement of NP-40 with CHAPS in isoelectric focusing of rainbow trout liver microsomes has increased resolution markedly. The two-dimensional electrophoretic system described will allow effective resolution of up to 300 μg of crude microsomal protein. CHAPS exhibits no effect on the stability or type of pH gradient when compared to NP-40 during isoelectric focusing in the presence of urea.

AB - CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-Propane sulfonate, a zwitterionic detergent, has been shown to exhibit superior membrane protein solubilizing characteristics as compared to nonionic detergents. Replacement of NP-40 with CHAPS in isoelectric focusing of rainbow trout liver microsomes has increased resolution markedly. The two-dimensional electrophoretic system described will allow effective resolution of up to 300 μg of crude microsomal protein. CHAPS exhibits no effect on the stability or type of pH gradient when compared to NP-40 during isoelectric focusing in the presence of urea.

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The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes

Abstract

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