TY - JOUR
T1 - The use of a zwitterionic detergent in two-dimensional gel electrophoresis of trout liver microsomes
AU - Perdew, Gary H.
AU - Schaup, Henry W.
AU - Selivonchick, Daniel P.
PY - 1983/12
Y1 - 1983/12
N2 - CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-Propane sulfonate, a zwitterionic detergent, has been shown to exhibit superior membrane protein solubilizing characteristics as compared to nonionic detergents. Replacement of NP-40 with CHAPS in isoelectric focusing of rainbow trout liver microsomes has increased resolution markedly. The two-dimensional electrophoretic system described will allow effective resolution of up to 300 μg of crude microsomal protein. CHAPS exhibits no effect on the stability or type of pH gradient when compared to NP-40 during isoelectric focusing in the presence of urea.
AB - CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-Propane sulfonate, a zwitterionic detergent, has been shown to exhibit superior membrane protein solubilizing characteristics as compared to nonionic detergents. Replacement of NP-40 with CHAPS in isoelectric focusing of rainbow trout liver microsomes has increased resolution markedly. The two-dimensional electrophoretic system described will allow effective resolution of up to 300 μg of crude microsomal protein. CHAPS exhibits no effect on the stability or type of pH gradient when compared to NP-40 during isoelectric focusing in the presence of urea.
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U2 - 10.1016/0003-2697(83)90711-X
DO - 10.1016/0003-2697(83)90711-X
M3 - Article
C2 - 6660519
AN - SCOPUS:0020997304
VL - 135
SP - 453
EP - 455
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -