The Use of Temperature Sensitivity and Selective Cell Culture Systems for Differentiation of Herpes Simplex Virus Types 1 and 2 in a Clinical Laboratory

J. J. Nordlund, C. Anderson, G. D. Hsiung, R. B. Tenser

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Abstract

A simple technique for differentiation of herpes simplex virus type 1 from type 2, using growth variations in tissue culture and temperature sensitivity is described. All HSV-2 isolates were capable of producing distinct microplaques in chick embryo cells while the newly isolated HSV-1 strains failed to do so. Newly isolated HSV-1 strains showed similar or slightly lower titers in rabbit kidney when incubated at 35 or 40°. In contrast, the HSV-2 isolates generally showed distinctly lower titers or delayed CPE at 40°. Virus typings by selective growth in cell culture and temperature markers were confirmed by neutralization and/or immunofluorescence tests. Since both types of HSV can be isolated from any site of the body, the availability of a simple laboratory technique for distinguishing between the two provides physicians and diagnostic virology laboratories with a means for rapid and accurate diagnosis of herpetic infections.

Original languageEnglish (US)
Pages (from-to)118-123
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume155
Issue number1
DOIs
Publication statusPublished - May 1977

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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